Category:MichSt14A 48

Figure 1C: Pictures of yeast plated. FLO11 gene controls hyphae (filaments) formation. WT grows hyphae in order to find more nutrients, while the knockout does not.

Figure 1A: Flocculation assay tests whether or not the cells aggregate together and drop to the bottom of the tube. The yeast does flocculate in the WT as the cells collect at the bottom. The knockout stays cloudy in the entire solution for the whole time.

Figure 1D: Adherence assay. Growing strain on plastic tests biofilm formation capabilities. There is growth seen on the WT plate but not with the knockout.

Figure 4 - Bead adhesion assay. Beads coated with the cells are placed on the plate to test cell-to-cell adhesion. In the WT, the growth is focused around the beads (black dots), while the growth in the knockout is not focused around the beads and is spread out around the plate.

Figure 1B showed the sigma1278b strain plated onto agar before and after washing. The WT could grow into the agar while the flo11 knockout could not