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User:Sainanij

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Phage Hunters Spring 2017

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
flaggedBPT4:HOLIN2017-04-23 22:57:36 CDTGO:0034291 canonical holin activity (F)PMID:11459934ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows the microscopic images of the bacterial cells pre and post induction of the Lambda lysis genes. Before the induction of the lysis genes, there was an abundant amount of bacterial cells present. After 25 minutes of the induction of the Lambda lysis genes, the amount of bacterial cells significantly decreased, showing that the cells were broken apart and lysed.

challenge
requireschangesBACSU:XHLB2017-04-29 19:39:21 CDTGO:0016021 integral component of membrane (C)PMID:9555893ECO:0000314 direct assay evidence used in manual assertion

PBSX is a late operon that consists of a gene that codes for a putative holin. Figure 2 of the article shows that the turbidity of the bacteria (at 550 nm) in the L8508 and POP1234 cultures decreased after the induction of PBSX, which contributes to the evidence for holin's function as lysis.

challenge

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Annotations challenged by Sainanij

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