CACAO Phage Hunters 2018
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| acceptable | BPT4:REPEA | 2018-03-04 19:10:41 CST | GO:0090592 DNA synthesis involved in DNA replication (P) | PMID:10559179 | ECO:0000315 mutant phenotype evidence used in manual assertion | repEA is an important gene in DNA synthesis and phage growth of phage T4. However, due to the amalgamation of several initiation sites in the T4 genome, mutations in repEA are not lethal. Nonsense mutations of repEA in conjunction with mot4 (to reduce initiation from different sources) mutations in phages showed smaller plaques compared to phages without both of these mutations (data not shown - from "Nonsense mutations in the repEA and repEB genes in combination with a motA mutation affect phage growth.")
The same repEA and mot4 mutation was shown to synthesize much less DNA compared to that of the phage with just the mot4 mutation (Figure 6), which was indicative of the importance of repEA versus other initiator proteins in DNA synthesis.
| challenge |
| acceptable | 9CAUD:Q94MV5 | 2018-03-26 11:41:16 CDT | GO:0008979 prophage integrase activity (F) | PMID:10383974 | ECO:0000314 direct assay evidence used in manual assertion | - CORRECTION FOR ROUND 2 LAMBD:VINT*
Figure 3 shows that without the int gene, site-specific integration does not occur. Plasmids that did not contain the int gene did not perform recombination in the attB locus. There are 2 alternate start codons within the coding sequence for the int gene that are essential for integrase function.
If there is a mutation in uoi, integrase still can function with the int gene. This proves that uoi is not required for integration in Mx8, but int is.
| challenge |
| acceptable | KLEPN:BLAN1 | 2018-04-01 19:38:32 CDT | GO:0008800 beta-lactamase activity (F) | PMID:19770275 | ECO:0000314 direct assay evidence used in manual assertion | Table 2 compares NDM-1's antibiotic kinetics to other metallo beta-lactamases in K. pneumoniae and shows that the New Delhi metallo beta-lactamase has very little in common comparatively to the other MBLs. Specifically that NDM-1 shows higher affinity for cephalosporins than IMP-1 and VIM-2 given by its lower Km value.
| challenge |
acceptable:3
unacceptable:0
requires_changes:0
flagged:0
Annotations challenged by SSingh
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
|---|
0 annotations fixed by SSingh
What do the icons mean in the status column?
