User:Rozwado5

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	XENLA:Q63ZQ7	2014-04-03 16:22:40 CDT	GO:2001032 regulation of double-strand break repair via nonhomologous end joining (P)	PMID:23324393	ECO:0000314 direct assay evidence used in manual assertion	Figure 1 shows that the SCF complex (Cul1 and Skp1) is required for Ku80 ubiquitylation. Ku80 is the initiating factor of nonhomologous end joining double-strand break repair. When polyubiquitylated, Ku80 dissociates from the DNA where the break was. Figure 4 (an immunoprecipitation) shows that when Fbxl2 is not present, the binding of Cul1 and Skp1 is inhibited and there are less modifications to Ku80. This proves that Fbx12 is the protein that recruits the SCF to double stranded breaks.	challenge
unacceptable	XENTR:A9JSE6	2014-04-05 18:12:27 CDT	GO:0003677 DNA binding (F)	PMID:23324393	ECO:0000314 direct assay evidence used in manual assertion	See Table 1 and Figure 2.	challenge
unacceptable	XENLA:Q63ZQ7	2014-04-05 18:18:39 CDT	GO:0003677 DNA binding (F)	PMID:23324393	ECO:0000314 direct assay evidence used in manual assertion	See Table 1 and Figure 2.	challenge
updatedbyinstructor	XENLA:Q7T0S5	2014-04-05 18:56:27 CDT	GO:0097443 sorting endosome (C)	PMID:24403139	ECO:0000314 direct assay evidence used in manual assertion	See Figure 4.	challenge
updatedbyinstructor	XENLA:Q9PWM7	2014-04-05 20:17:35 CDT	GO:1900087 positive regulation of G1/S transition of mitotic cell cycle (P)	PMID:24403013	ECO:0000315 mutant phenotype evidence used in manual assertion	See Figure 1	challenge
unacceptable	HUMAN:OGFD1	2014-04-05 21:28:45 CDT	GO:0043022 ribosome binding (F)	PMID:24550447	ECO:0000314 direct assay evidence used in manual assertion	See Figure 3. In A, a 100-kDa species in an immunoprecipitation is consistent with the weight of a hypothetical OGFOD1-RPS23 complex. Other experiments on this figure support this. RPS23 is a ribosomal binding site.	challenge
acceptable	HUMAN:OGFD1	2014-04-05 21:28:46 CDT	GO:0006449 regulation of translational termination (P)	PMID:24550447	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5 shows that OGFOD1 knockouts are less likely to terminate at a nonsense codon. This implies that OGFOD1 modulates translation termination efficiency. A catalytically inactive mutant also terminates earlier, similar to the knockout. This suggests that it is the ribosomal binding function of OGFOD1 that regulates the translational termination.	challenge
updatedbyinstructor	HUMAN:OGFD1	2014-04-05 22:05:56 CDT	GO:0019511 peptidyl-proline hydroxylation (P)	PMID:24550447	ECO:0000314 direct assay evidence used in manual assertion	Figure S4A shows that OGFOD1 does in vitro hydroxylation of a peptide derived from RPS23, assayed by mass spec.	challenge

acceptable:2
unacceptable:3
requires_changes:0
flagged:0

Annotations challenged by Rozwado5

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Rozwado5

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