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User:Parimaj1

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Phage Hunters Spring 2016

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorBPSPP:PORTL2016-04-10 02:35:21 CDTGO:0098006 viral DNA genome packaging, headful (P)PMID:15110523ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2 demonstrates gp6 single amino acid substitutions affecting the packaging of sizX chromosome.

challenge
unacceptableBPSPP:PORTL2016-04-06 09:06:14 CDTGO:0046798 viral portal complex (C)PMID:8467790ECO:0000314 direct assay evidence used in manual assertion

"In this model one full 3600 rotation of the portal protein corresponds to inserting 65 bp of dsDNA at the expense of a minimum of 32.5 ATPs, if it is assumed that each equivalent unitary interaction between the two structures is coupled to one ATP hydrolysis reaction and leads to the translocation of 2 bp (Figure 3d-e). Furthermore, the model predicts (Figure 4) that the portal protein subunits are activated in the order: 1, 4, 7, 10, 13, 3, 6, 9, 12, 2, 5, 8, 11, 1, etc., thereby equally employing all subunits of the portal structure during the translocation process."

challenge
unacceptableBPT4:PORTL2016-04-11 09:24:12 CDTGO:0019073 viral DNA genome packaging (P)PMID:10366503ECO:0000314 direct assay evidence used in manual assertion

"In any case, a scrambled 24 residue peptide with the same amino acid composition (Figure 1(c)) was without effect on in vitropackaging by the same assay, thereby providing evidence, at least with the unpolymerized peptide, that the packaging inhibition observed was specific and dependent upon the gp20 peptide sequence"

challenge
unacceptableBPSPP:PORTL2016-04-10 02:41:53 CDTGO:0019073 viral DNA genome packaging (P)PMID:15110523ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 demonstrates "Arrowheads show the mature DNA produced upon complementation with the sizX allele (1), mature DNA resulting from background effects under our experimental conditions (see text) (2), DNA packaged in presence of gp6 mutant forms that caused a low efficiency phenotype (3), and DNA packaged in presence of a gp6Siz mutant protein (4)."

challenge

acceptable:0
unacceptable:3
requires_changes:0
flagged:0

Annotations challenged by Parimaj1

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
unacceptableOmololu1,
Team Blue B
9CAUD:A0A0K2CZZ6GO:0031072 - heat shock protein binding (F)PMID:2527744ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 5

0 annotations fixed by Parimaj1

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