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BPSPP:PORTL
Contents
Species (Taxon ID) | Bacillus phage SPP1 (Bacteriophage SPP1). (10724) | |
Gene Name(s) | 6 (synonyms: siz) | |
Protein Name(s) | Portal protein
GP6 Portal vertex protein | |
External Links | ||
UniProt | P54309 | |
EMBL | X56064 X97918 | |
PIR | S21805 | |
RefSeq | NP_690661.1 | |
PDB | 2JES | |
PDBsum | 2JES | |
ProteinModelPortal | P54309 | |
SMR | P54309 | |
GeneID | 955279 | |
EvolutionaryTrace | P54309 | |
Proteomes | UP000002559 | |
GO | GO:0046729 GO:0019012 | |
InterPro | IPR021145 IPR006428 | |
Pfam | PF05133 | |
TIGRFAMs | TIGR01538 |
Annotations
Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|---|---|
GO:0019012 |
virion |
ECO:0000314 |
C |
Figure 2. |
complete | |||||
GO:0046729 |
viral procapsid |
ECO:0000314 |
C |
Figure 2. |
complete | |||||
GO:0019012 |
virion |
ECO:0000314 |
C |
Figure 4-A. |
complete | |||||
GO:0098006 |
viral DNA genome packaging, headful |
ECO:0000315 |
P |
Table 2 demonstrates gp6 single amino acid substitutions affecting the packaging of sizX chromosome. |
complete | |||||
GO:0046798 |
viral portal complex |
ECO:0000314 |
C |
"In this model one full 3600 rotation of the portal protein corresponds to inserting 65 bp of dsDNA at the expense of a minimum of 32.5 ATPs, if it is assumed that each equivalent unitary interaction between the two structures is coupled to one ATP hydrolysis reaction and leads to the translocation of 2 bp (Figure 3d-e). Furthermore, the model predicts (Figure 4) that the portal protein subunits are activated in the order: 1, 4, 7, 10, 13, 3, 6, 9, 12, 2, 5, 8, 11, 1, etc., thereby equally employing all subunits of the portal structure during the translocation process." |
complete | |||||
GO:0019073 |
viral DNA genome packaging |
ECO:0000315 |
P |
Figure 3 demonstrates "Arrowheads show the mature DNA produced upon complementation with the sizX allele (1), mature DNA resulting from background effects under our experimental conditions (see text) (2), DNA packaged in presence of gp6 mutant forms that caused a low efficiency phenotype (3), and DNA packaged in presence of a gp6Siz mutant protein (4)." |
complete | |||||
GO:0046729 |
viral procapsid |
ECO:0000314 |
C |
Figure 3 indicates that gp13 within phage SPP1 functions as a major capsid protein. The protein composition of purified procapsids was checked by SDS-PAGE stained with Coomassie blue to confirm the presence of the major procapsid proteins gp11 (scaffolding protein) and gp13 (major capsid protein). |
complete | |||||
part_of |
GO:0046798 |
viral portal complex |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
part_of |
GO:0046729 |
viral procapsid |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
part_of |
GO:0019012 |
virion |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
part_of |
GO:0019012 |
virion |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
involved_in |
GO:0099001 |
viral genome ejection through host cell envelope, long flexible tail mechanism |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
part_of |
GO:0019028 |
viral capsid |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
part_of |
GO:0019012 |
virion |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
involved_in |
GO:0046718 |
viral entry into host cell |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Vinga, I et al. (2006) The minor capsid protein gp7 of bacteriophage SPP1 is required for efficient infection of Bacillus subtilis. Mol. Microbiol. 61 1609-21 PubMed GONUTS page
- ↑ 2.0 2.1 2.2 2.3 Isidro, A et al. (2004) The high-resolution functional map of bacteriophage SPP1 portal protein. Mol. Microbiol. 51 949-62 PubMed GONUTS page
- ↑ 3.0 3.1 Becker, B et al. (1997) Head morphogenesis genes of the Bacillus subtilis bacteriophage SPP1. J. Mol. Biol. 268 822-39 PubMed GONUTS page
- ↑ 4.0 4.1 Isidro, A et al. (2004) The portal protein plays essential roles at different steps of the SPP1 DNA packaging process. Virology 322 253-63 PubMed GONUTS page
- ↑ Dube, P et al. (1993) The portal protein of bacteriophage SPP1: a DNA pump with 13-fold symmetry. EMBO J. 12 1303-9 PubMed GONUTS page