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BPSPP:PORTL

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Species (Taxon ID) Bacillus phage SPP1 (Bacteriophage SPP1). (10724)
Gene Name(s) 6 (synonyms: siz)
Protein Name(s) Portal protein

GP6 Portal vertex protein

External Links
UniProt P54309
EMBL X56064
X97918
PIR S21805
RefSeq NP_690661.1
PDB 2JES
PDBsum 2JES
ProteinModelPortal P54309
SMR P54309
GeneID 955279
EvolutionaryTrace P54309
Proteomes UP000002559
GO GO:0046729
GO:0019012
InterPro IPR021145
IPR006428
Pfam PF05133
TIGRFAMs TIGR01538

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0019012

virion

PMID:16899078[1]

ECO:0000314

C

Figure 2.

complete
CACAO 6842

GO:0046729

viral procapsid

PMID:14763972[2]

ECO:0000314

C

Figure 2.

complete
CACAO 6844

GO:0019012

virion

PMID:9180375[3]

ECO:0000314

C

Figure 4-A.

complete
CACAO 6861

GO:0098006

viral DNA genome packaging, headful

PMID:15110523[4]

ECO:0000315

P

Table 2 demonstrates gp6 single amino acid substitutions affecting the packaging of sizX chromosome.

complete
CACAO 11418

GO:0046798

viral portal complex

PMID:8467790[5]

ECO:0000314

C

"In this model one full 3600 rotation of the portal protein corresponds to inserting 65 bp of dsDNA at the expense of a minimum of 32.5 ATPs, if it is assumed that each equivalent unitary interaction between the two structures is coupled to one ATP hydrolysis reaction and leads to the translocation of 2 bp (Figure 3d-e). Furthermore, the model predicts (Figure 4) that the portal protein subunits are activated in the order: 1, 4, 7, 10, 13, 3, 6, 9, 12, 2, 5, 8, 11, 1, etc., thereby equally employing all subunits of the portal structure during the translocation process."

complete
CACAO 11419

GO:0019073

viral DNA genome packaging

PMID:15110523[4]

ECO:0000315

P

Figure 3 demonstrates "Arrowheads show the mature DNA produced upon complementation with the sizX allele (1), mature DNA resulting from background effects under our experimental conditions (see text) (2), DNA packaged in presence of gp6 mutant forms that caused a low efficiency phenotype (3), and DNA packaged in presence of a gp6Siz mutant protein (4)."

complete
CACAO 11556

GO:0046729

viral procapsid

PMID:14763972[2]

ECO:0000314

C

Figure 3 indicates that gp13 within phage SPP1 functions as a major capsid protein. The protein composition of purified procapsids was checked by SDS-PAGE stained with Coomassie blue to confirm the presence of the major procapsid proteins gp11 (scaffolding protein) and gp13 (major capsid protein).

complete
CACAO 12922

part_of

GO:0046798

viral portal complex

PMID:14763972[2]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0046729

viral procapsid

PMID:14763972[2]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0019012

virion

PMID:9180375[3]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0019012

virion

PMID:16899078[1]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

involved_in

GO:0099001

viral genome ejection through host cell envelope, long flexible tail mechanism

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1243

P

Seeded From UniProt

complete

part_of

GO:0019028

viral capsid

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0167

C

Seeded From UniProt

complete

part_of

GO:0019012

virion

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0946
UniProtKB-SubCell:SL-0274

C

Seeded From UniProt

complete

involved_in

GO:0046718

viral entry into host cell

GO_REF:0000037
GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1160
UniProtKB-KW:KW-1162

P

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 Vinga, I et al. (2006) The minor capsid protein gp7 of bacteriophage SPP1 is required for efficient infection of Bacillus subtilis. Mol. Microbiol. 61 1609-21 PubMed GONUTS page
  2. 2.0 2.1 2.2 2.3 Isidro, A et al. (2004) The high-resolution functional map of bacteriophage SPP1 portal protein. Mol. Microbiol. 51 949-62 PubMed GONUTS page
  3. 3.0 3.1 Becker, B et al. (1997) Head morphogenesis genes of the Bacillus subtilis bacteriophage SPP1. J. Mol. Biol. 268 822-39 PubMed GONUTS page
  4. 4.0 4.1 Isidro, A et al. (2004) The portal protein plays essential roles at different steps of the SPP1 DNA packaging process. Virology 322 253-63 PubMed GONUTS page
  5. Dube, P et al. (1993) The portal protein of bacteriophage SPP1: a DNA pump with 13-fold symmetry. EMBO J. 12 1303-9 PubMed GONUTS page