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User:Nwang4

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Phage Hunters Spring 2016

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableHUMAN:PGK12016-03-28 10:07:26 CDTGO:0005739 mitochondrion (C)PMID:26942675ECO:0000314 direct assay evidence used in manual assertion

Cell fractionation shows that cytosolic PGK1 translocates to the mitochondria in response to hypoxia (Figure S1B).

challenge
acceptableBACSU:YNDL2016-03-28 11:04:38 CDTGO:0016787 hydrolase activity (F)PMID:26158264ECO:0000314 direct assay evidence used in manual assertion

Enzyme assay viewed by gel separation shows that YndL reduces the molecular weight of y-PGA over time (Fig 3A), providing evidence that YndL is a y-PGA hydrolase.

challenge
acceptableBACSU:YOQZ2016-03-28 11:09:03 CDTGO:0016787 hydrolase activity (F)PMID:26158264ECO:0000314 direct assay evidence used in manual assertion

Enzyme assay viewed by gel separation shows that YoqZ reduces the molecular weight of y-PGA over time (Fig 3A), providing evidence that YoqZ is a y-PGA hydrolase.

challenge
unacceptable9VIRU:Q5ILC62016-04-11 04:58:45 CDTGO:0003700 transcription factor activity, sequence-specific DNA binding (F)PMID:26138485ECO:0000315 mutant phenotype evidence used in manual assertion

In mutant SOS-deficient LexA hosts, transcription of the P1-P2-lacZ fusion gene, which is otherwise induced during SOS conditions, was not activated. Thus, LexA regulates transcription.

Mutations in SOS box dinbox1 (a region on DNA), known to be important for LexA binding, makes it so that SOS can no longer be induced (Fig 1B), showing that LexA, which causes SOS induction, binds to dinbox1.

challenge

acceptable:2
unacceptable:2
requires_changes:0
flagged:0

Annotations challenged by Nwang4

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
unacceptableOmololu1,
Team Blue B
9CAUD:A0A0K2CZZ6GO:0031072 - heat shock protein binding (F)PMID:2527744ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 5
unacceptableOmololu1,
Team Blue B
9CAUD:A0A0K2D0J0GO:0000166 - nucleotide binding (F)PMID:17209017ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 3

0 annotations fixed by Nwang4

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