User:Njd5204

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	ECOLI:CSRA	2015-09-20 15:33:01 CDT	GO:0048255 mRNA stabilization (P)	PMID:23305111	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 6, mRNA half-life reduced in csrA mutant compared to WT	challenge
acceptable	ECOLI:CSRA	2015-10-14 09:48:41 CDT	GO:0031438 negative regulation of mRNA cleavage (P)	PMID:23305111	ECO:0000314 direct assay evidence used in manual assertion	Figure 5A, in vitro experiment using purified RNase E. In the presence of CsrA, the RNA was protected from RNase E cleavage.	challenge
unacceptable	BACSU:MTRB	2015-10-28 13:16:00 CDT	GO:0031555 transcriptional attenuation (P)	PMID:7592410	ECO:0000314 direct assay evidence used in manual assertion	Figure 4, in vitro experiment using a complementary oligo to the DNA terminator hairpin in a transcription assay, showing a decrease in % termination, which supports the developed model of attenuation done by TRAP	challenge
acceptable	BACSU:MTRB	2015-10-28 13:21:42 CDT	GO:0060566 positive regulation of DNA-templated transcription, termination (P)	PMID:7592410	ECO:0000314 direct assay evidence used in manual assertion	Figure 3. In vitro transcription assay, in the presence of TRAP and L-tryptophan transcription termination greatly increases.	challenge
acceptable	BACSU:MTRB	2015-10-28 13:59:01 CDT	GO:0045947 negative regulation of translational initiation (P)	PMID:9098056	ECO:0000314 direct assay evidence used in manual assertion	Figure 5. Toeprint assay showing TRAP binding near the Shine-Dalgarno and overlapping with the 30S subunit. Figure 6. In vitro translation using trpG mRNA and increasing amounts of TRAP, showing reduced translation in the presence of TRAP.	challenge
acceptable	BACSU:MTRB	2015-10-28 14:16:51 CDT	GO:0045947 negative regulation of translational initiation (P)	PMID:14702295	ECO:0000314 direct assay evidence used in manual assertion	Figure 5. Toeprint assay showing overlapping 30S ribosome and TRAP binding sites. Figure 6. in vitro translation showing a decrease in translation of the trpP'-'gfp mRNA with increasing concentrations of TRAP.	challenge

acceptable:5
unacceptable:1
requires_changes:0
flagged:0

Annotations challenged by Njd5204

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Njd5204

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