GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
BACSU:MTRB
Contents
| Species (Taxon ID) | Bacillus subtilis (strain 168). (224308) | |
| Gene Name(s) | mtrB | |
| Protein Name(s) | Transcription attenuation protein MtrB
Trp RNA-binding attenuation protein TRAP Tryptophan RNA-binding attenuator protein | |
| External Links | ||
| UniProt | P19466 | |
| EMBL | M37320 M80245 AL009126 | |
| PIR | B38256 | |
| RefSeq | NP_390158.1 WP_003230576.1 | |
| PDB | 1WAP 3ZZQ 4B27 | |
| PDBsum | 1WAP 3ZZQ 4B27 | |
| ProteinModelPortal | P19466 | |
| STRING | 224308.Bsubs1_010100012511 | |
| EnsemblBacteria | CAB14193 | |
| GeneID | 23410310 938996 | |
| KEGG | bsu:BSU22770 | |
| PATRIC | 18976365 | |
| GenoList | BSU22770 | |
| HOGENOM | HOG000245485 | |
| KO | K06285 | |
| OMA | FHHTEKL | |
| OrthoDB | EOG64V2JJ | |
| BioCyc | BSUB:BSU22770-MONOMER | |
| EvolutionaryTrace | P19466 | |
| Proteomes | UP000001570 | |
| GO | GO:0003723 GO:0006353 GO:0006355 | |
| Gene3D | 2.60.40.50 | |
| HAMAP | MF_00798 | |
| InterPro | IPR000824 IPR016031 IPR023558 | |
| Pfam | PF02081 | |
| PRINTS | PR00687 | |
| SUPFAM | SSF51219 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0031555 |
transcriptional attenuation |
ECO:0000314 |
P |
Figure 4, in vitro experiment using a complementary oligo to the DNA terminator hairpin in a transcription assay, showing a decrease in % termination, which supports the developed model of attenuation done by TRAP |
complete | |||||
| GO:0060566 |
positive regulation of DNA-templated transcription, termination |
ECO:0000314 |
P |
Figure 3. In vitro transcription assay, in the presence of TRAP and L-tryptophan transcription termination greatly increases. |
complete | |||||
|
enables |
GO:0003723 |
RNA binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0045947 |
negative regulation of translational initiation |
ECO:0000314 |
P |
Figure 5. Toeprint assay showing TRAP binding near the Shine-Dalgarno and overlapping with the 30S subunit. Figure 6. In vitro translation using trpG mRNA and increasing amounts of TRAP, showing reduced translation in the presence of TRAP. |
complete | |||||
|
involved_in |
GO:0006353 |
DNA-templated transcription, termination |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0045947 |
negative regulation of translational initiation |
ECO:0000314 |
P |
Figure 5. Toeprint assay showing overlapping 30S ribosome and TRAP binding sites. Figure 6. in vitro translation showing a decrease in translation of the trpP'-'gfp mRNA with increasing concentrations of TRAP. |
complete | |||||
|
involved_in |
GO:0006355 |
regulation of transcription, DNA-templated |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003723 |
RNA binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000033380 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003723 |
RNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Merino, E et al. (1995) trp RNA-binding attenuation protein (TRAP)-trp leader RNA interactions mediate translational as well as transcriptional regulation of the Bacillus subtilis trp operon. J. Bacteriol. 177 6362-70 PubMed GONUTS page
- ↑ Du, H et al. (1997) The trp RNA-binding attenuation protein regulates TrpG synthesis by binding to the trpG ribosome binding site of Bacillus subtilis. J. Bacteriol. 179 2582-6 PubMed GONUTS page
- ↑ Yakhnin, H et al. (2004) The trp RNA-binding attenuation protein of Bacillus subtilis regulates translation of the tryptophan transport gene trpP (yhaG) by blocking ribosome binding. J. Bacteriol. 186 278-86 PubMed GONUTS page
