GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

User:Mtaya1

From GONUTS
Jump to: navigation, search

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesBORPE:BVGA2012-09-23 13:53:18 CDTGO:0000156 two-component response regulator activity (F)PMID:16238621ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5. shows that the T194M mutant had a significantly reduced rate of phosphorylation by the BvgS sensor kinase.

challenge
requireschangesBORPE:BVGA2012-09-23 13:29:59 CDTGO:0001140 bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription (F)PMID:16238621ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 8. compares the ability of WT and mutant BvgA protein to activate transcription by binding to two promoters, in the presence of RNA Polymerase.

challenge
acceptableBORPE:BVGA2012-09-23 13:52:40 CDTGO:0009405 pathogenesis (P)PMID:16238621ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2. shows that two BvgA mutants, EMS12 and EMS24, had failed to express the Bvg+ phase phenotype necessary for virulence.

challenge
requireschangesBORBR:BVGS2012-09-23 15:00:59 CDTGO:0009405 pathogenesis (P)PMID:9194696ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1. shows several BvgS mutants with reduced virulence (Bvg- or Bvgi).

challenge
acceptableECOLI:RUTA2012-11-18 16:58:05 CSTGO:0052614 uracil oxygenase activity (F)PMID:20400551ECO:0000314 direct assay evidence used in manual assertion

Figure 3. shows NMR evidence that RutA cleaves uracil between N-3 and C-4 and incorporates oxygen from molecular oxygen.

challenge
acceptableECOLI:RUTA2012-11-18 16:23:22 CSTGO:0006212 uracil catabolic process (P)PMID:20400551ECO:0000314 direct assay evidence used in manual assertion

Figure 2B. shows the disappearance of 14C-labeled uracil at the C-2 and C-6 positions due to action by RutA. The product of RutA is at a different position on the TLC plate compared with the substrate uracil.

challenge
acceptableECOLI:RUTA2012-11-18 16:26:47 CSTGO:0006210 thymine catabolic process (P)PMID:20400551ECO:0000314 direct assay evidence used in manual assertion

Figure 2C. shows the disappearance of 14C-labeled thymine at the C-2 and C-6 positions due to action by RutA. The product of RutA is at a different location on the TLC plate compared with the thymine substrate.

challenge
acceptableECOLI:RUTB2012-11-18 17:38:56 CSTGO:0006212 uracil catabolic process (P)PMID:20400551ECO:0000314 direct assay evidence used in manual assertion

Figure 2B. shows the disappearance of the RutA product (3-ureidoacrylate) by RutB.

challenge

acceptable:5
unacceptable:0
requires_changes:3
flagged:0

Annotations challenged by Mtaya1

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Mtaya1