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ECOLI:RUTA

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Species (Taxon ID) Escherichia coli (strain K12). (83333)
Gene Name(s) rutA (synonyms: ycdM)
Protein Name(s) Pyrimidine monooxygenase RutA
External Links
UniProt P75898
EMBL U00096
AP009048
PIR B64843
RefSeq NP_415532.3
YP_489285.1
ProteinModelPortal P75898
SMR P75898
IntAct P75898
STRING 511145.b1012
EnsemblBacteria AAC74097
BAA35789
GeneID 12931044
945643
KEGG ecj:Y75_p0985
eco:b1012
PATRIC 32117253
EchoBASE EB3619
EcoGene EG13859
eggNOG COG2141
HOGENOM HOG000268769
InParanoid P75898
KO K09018
OMA ADYNFCF
OrthoDB EOG6RNQ9G
PhylomeDB P75898
BioCyc EcoCyc:G6523-MONOMER
ECOL316407:JW0997-MONOMER
MetaCyc:G6523-MONOMER
PRO PR:P75898
Proteomes UP000000318
UP000000625
Genevestigator P75898
GO GO:0004497
GO:0016705
GO:0052614
GO:0019740
GO:0006208
GO:0006210
GO:0006212
Gene3D 3.20.20.30
HAMAP MF_01699
InterPro IPR011251
IPR019914
Pfam PF00296
SUPFAM SSF51679
TIGRFAMs TIGR03612

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0052614

uracil oxygenase activity

PMID:20400551[1]

ECO:0000314

F

Figure 3. shows NMR evidence that RutA cleaves uracil between N-3 and C-4 and incorporates oxygen from molecular oxygen.

complete
CACAO 6241

GO:0006212

uracil catabolic process

PMID:20400551[1]

ECO:0000314

P

Figure 2B. shows the disappearance of 14C-labeled uracil at the C-2 and C-6 positions due to action by RutA. The product of RutA is at a different position on the TLC plate compared with the substrate uracil.

complete
CACAO 6247

GO:0006210

thymine catabolic process

PMID:20400551[1]

ECO:0000314

P

Figure 2C. shows the disappearance of 14C-labeled thymine at the C-2 and C-6 positions due to action by RutA. The product of RutA is at a different location on the TLC plate compared with the thymine substrate.

complete
CACAO 6251

enables

GO:0052614

uracil oxygenase activity

PMID:20400551[1]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0006212

uracil catabolic process

PMID:20400551[1]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0006210

thymine catabolic process

PMID:20400551[1]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0019740

nitrogen utilization

PMID:16540542[2]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0006212

uracil catabolic process

PMID:20400551[1]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0004497

monooxygenase activity

PMID:20400551[1]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0052614

uracil oxygenase activity

PMID:20369853[3]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0019740

nitrogen utilization

PMID:16540542[2]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0006212

uracil catabolic process

PMID:20400551[1]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0006208

pyrimidine nucleobase catabolic process

PMID:16540542[2]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0004497

monooxygenase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR019914

F

Seeded From UniProt

complete

involved_in

GO:0006212

uracil catabolic process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR019914

P

Seeded From UniProt

complete

enables

GO:0016705

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR011251
InterPro:IPR036661

F

Seeded From UniProt

complete

involved_in

GO:0055114

oxidation-reduction process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR011251
InterPro:IPR019914
InterPro:IPR036661

P

Seeded From UniProt

complete

enables

GO:0004497

monooxygenase activity

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000084167

F

Seeded From UniProt

complete

involved_in

GO:0006212

uracil catabolic process

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000084167

P

Seeded From UniProt

complete

involved_in

GO:0019740

nitrogen utilization

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000084167

P

Seeded From UniProt

complete

enables

GO:0016491

oxidoreductase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0560

F

Seeded From UniProt

complete

involved_in

GO:0055114

oxidation-reduction process

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0560

P

Seeded From UniProt

complete

enables

GO:0004497

monooxygenase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0503

F

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Kim, KS et al. (2010) The Rut pathway for pyrimidine degradation: novel chemistry and toxicity problems. J. Bacteriol. 192 4089-102 PubMed GONUTS page
  2. 2.0 2.1 2.2 Loh, KD et al. (2006) A previously undescribed pathway for pyrimidine catabolism. Proc. Natl. Acad. Sci. U.S.A. 103 5114-9 PubMed GONUTS page
  3. Mukherjee, T et al. (2010) Catalysis of a flavoenzyme-mediated amide hydrolysis. J. Am. Chem. Soc. 132 5550-1 PubMed GONUTS page