User:Mammela1

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	STRLN:Q54354	2012-04-12 11:56:29 CDT	GO:0046566 DOPA dioxygenase activity (F)	PMID:15355345	ECO:0000314 direct assay evidence used in manual assertion	Fig 5: NMR was used to determine the biosynthetic origin of carbon and nitrogen atoms in a lincomycin molecule. This figure provides evidence of 2,3-extradiol cleavage of the L-DOPA aromatic ring by LmbB1.	challenge
acceptable	SYNY3:BQMT	2012-04-14 19:57:01 CDT	GO:0051741 2-methyl-6-phytyl-1,4-benzoquinone methyltransferase activity (F)	PMID:11821038	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 3: Shows differences in tocophenol composition compared to the wildtype. The mutant contained a significant amount of alpha and beta tocophenol. Showing that the MPBQ methyltransferase appears to play a more important role in determining tocophenol composition than it does in tocophenol content.	challenge
requireschanges	BRAOL:TMT2	2012-04-14 22:50:54 CDT	GO:0018708 thiol S-methyltransferase activity (F)	PMID:12369626	ECO:0000270 expression pattern evidence used in manual assertion	Table 2: Shows the ability of TMT to methylate various substrates. The greatest activity observed was for the substrates containing thio groups. Fig 5: Shows that TMT activity is especially high in younger tissues. The high amount of glycosinolate that results provides defense against herbivores.	challenge
requireschanges	PEA:G2OX1	2012-04-14 23:31:28 CDT	GO:0045543 gibberellin 2-beta-dioxygenase activity (F)	PMID:16181493	ECO:0000314 direct assay evidence used in manual assertion	Fig 5: The pure protein's enzymatic activity was measured using GA(20) as a substrate. The Michealis-Menton plot shows that GA2OX1 is the main catalyst for the conversion of GA(20) by 2-oxidation	challenge
requireschanges	ORYSJ:Q8LQQ2	2012-04-15 15:49:42 CDT	GO:0010437 9,10 (9', 10')-carotenoid-cleaving dioxygenase activity (F)	PMID:19120446	ECO:0000314 direct assay evidence used in manual assertion	Fig 5: OsCCD1 exhibited by far the highest preference for the C9-C10 double bond in vitro. As suggested by the predominance of the C(17) dialdehye (90% of the total dialdehyde products)	challenge
acceptable	CERPU:A7UFH0	2012-04-15 16:31:05 CDT	GO:0016213 linoleoyl-CoA desaturase activity (F)	PMID:10848999	ECO:0000314 direct assay evidence used in manual assertion	Fig 3: Show that mass spectra was used to determine that the isolated fatty-acid products were desaturated in the delta(6) position. This was confirmed using NMR.	challenge

acceptable:2
unacceptable:0
requires_changes:4
flagged:0

Annotations challenged by Mammela1

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Mammela1

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	PLAF7:Q8IL04	2012-03-29 12:54:48 CDT	GO:0004392 heme oxygenase (decyclizing) activity (F)	PMID:18437218	ECO:0000314 direct assay evidence used in manual assertion	Figure 5b - immuno-fluorescence staining using an anti-HDP antibody showed that the transfected parasite continually expressed HDP, this indicated that the HDP locus was not disrupted.	challenge

acceptable:0
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Mammela1

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Mammela1