GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:11821038
| Citation |
Shintani, DK, Cheng, Z and DellaPenna, D (2002) The role of 2-methyl-6-phytylbenzoquinone methyltransferase in determining tocopherol composition in Synechocystis sp. PCC6803. FEBS Lett. 511:1-5 |
|---|---|
| Abstract |
A putative 2-methyl-6-phytylbenzoquinone (MPBQ) methyltransferase gene, SLL0418, was identified from the Synechocystis PCC6803 genome based on its homology to previously characterized gamma-tocopherol methyltransferases. Genetic and biochemical evidence confirmed open reading frame (ORF) SLL0418 encodes a MPBQ methyltransferase. An SLL0418 partial knockout mutant accumulated beta-tocopherol with no effect in the overall tocopherol content of the cell. In vitro assays of the SLL0418 gene expressed in Escherichia coli showed the enzyme efficiently catalyzes methylation of ring carbon 3 of MPBQ. In addition, the enzyme also catalyzes the methylation of ring carbon 3 of 2-methyl-6-solanylbenzoquinol in the terminal step of plastoquinone biosynthesis. |
| Links | |
| Keywords |
Amino Acid Sequence; Chromatography, High Pressure Liquid; Cyanobacteria/chemistry; Cyanobacteria/enzymology; Cyanobacteria/genetics; Gas Chromatography-Mass Spectrometry; Methylation; Methyltransferases/chemistry; Methyltransferases/genetics; Methyltransferases/metabolism; Molecular Sequence Data; Mutagenesis, Site-Directed; Plastoquinone/analogs & derivatives; Plastoquinone/metabolism; Sequence Homology, Amino Acid; Time Factors; Tocopherols/analysis; Tocopherols/chemistry; Tocopherols/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0051741: 2-methyl-6-phytyl-1,4-benzoquinone methyltransferase activity |
ECO:0000315: |
F |
Fig 3: Shows differences in tocophenol composition compared to the wildtype. The mutant contained a significant amount of alpha and beta tocophenol. Showing that the MPBQ methyltransferase appears to play a more important role in determining tocophenol composition than it does in tocophenol content. |
complete | ||||
|
enables |
GO:0051741: 2-methyl-6-phytyl-1,4-benzoquinone methyltransferase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
See Help:References for how to manage references in GONUTS.
