User:JacobChamblee

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	BPT4:DMD	2016-04-30 23:36:59 CDT	GO:1903902 positive regulation of viral life cycle (P)	PMID:22403819	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 7A shows inhibited plaquing by T4 dmd knockouts compared to WT.	challenge
unacceptable	BPMU:NER	2016-04-30 23:39:26 CDT	GO:0042262 DNA protection (P)	PMID:2537762	ECO:0000314 direct assay evidence used in manual assertion	Figure 3A shows protection of DNA from DNAse I hydrolysis.	challenge
updatedbyinstructor	BPP4:VINT	2016-05-01 00:11:04 CDT	GO:0039695 DNA-templated viral transcription (P)	PMID:16847139	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3 shows increasingly inhibited Int promoter transcription as increasingly larger fractions of the P4 Int gene are expressed. "Thus, when the P4 DNA fragment contained the whole int gene (pGM606) or at least 60 % of it (pGM605), the int mRNA abundance was greatly reduced, suggesting that Int negatively regulates transcription initiation at P int."	challenge
updatedbyinstructor	9CAUD:I1TEH8	2016-05-01 18:40:23 CDT	GO:0044659 cytolysis by virus of host cell (P)	PMID:24185850	ECO:0000316 genetic interaction evidence used in manual assertion	Fig 4B indicates holin activity by the presence of a sudden cell growth plateau via lysis curve (closed black circle).	challenge
updatedbyinstructor	BPP22:SIEA	2016-05-01 18:57:11 CDT	GO:0033644 host cell membrane (C)	PMID:7768804	ECO:0000314 direct assay evidence used in manual assertion	Figure 3 shows a hydropathic plot for sieA and indicates that sieA is a membrane protein. NaOH fractionation (Figure 5) indicates the presence of sieA in the insoluble membrane fraction. sieA is not detectable in the soluble fractions.	challenge
acceptable	BPP2:BPX	2016-05-02 20:08:17 CDT	GO:0098025 virus tail, baseplate (C)	PMID:24097944	ECO:0000314 direct assay evidence used in manual assertion	Figure 1 shows localization of MBP-tagged gpX to the baseplate of the phage. The higher density observed compared to WT is a consequence of the 367 residue MBP tag on plasmid expressed gpX.	challenge

acceptable:1
unacceptable:2
requires_changes:0
flagged:0

Annotations challenged by JacobChamblee

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by JacobChamblee

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