User:Culpepperr

Phage Hunters Spring 2017

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
flagged	BPPRD:HOLIN	2017-04-12 11:14:20 CDT	GO:0019835 cytolysis (P)	PMID:12813073	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3a. shows Phage PRD1 sus715 amber mutant shows loss of cytolysis phenotype is restored with bacteriophage lambda gene S105. The filled circle line shows sus715 mutant without induction - showing the lack of lysis with the mutant. The filled square shows the sus715 mutant in the presence of lambda holin induced with the addition of a sugar IPTG, and cytolysis is restored. The filled triangle shows the wild type phage and how they would expect the gene to respond without a mutation.	challenge
flagged	ECOLI:CH10	2017-04-26 09:22:10 CDT	GO:0019835 cytolysis (P)	PMID:16030234	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4 shows E. coli with mutations to groES that impact the ability of phage PRD1 to lyse the host bacteria. Temperature sensitive mutants, closed triangle, groES mutant DW719(pLM2), lost cytolysis at 40 degrees. The wt closed circle wt strain DW720(pLM2), does not lose cytolysis at 40 degrees. This shows loss of cytolysis when the temperature is raised with this gene and the normal lysis in the wt as control.	challenge

acceptable:0
unacceptable:0
requires_changes:0
flagged:2

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Culpepperr