User:Canova1

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	9CAUD:A0A125QYI2	2017-04-05 10:15:53 CDT	GO:0030308 negative regulation of cell growth (P)	PMID:27725812	ECO:0000314 direct assay evidence used in manual assertion	Figure 1: Analyzed the growth curve based on cell density at 600nm (Figure 1C), and colony-forming unit calculations (Figure 1D) to identify the effect of gp70.1 (from the Bacteriophage PaP3) on P. aeruginosa PA3. The black circles on the graphs indicate the PA3 cells with the gp 70.1 gene; they had decreased cell density (1C) and decreased growth rate (1D); therefore, it was determined that Gp70.1 had a severe inhibitory effect on the concentration of bacterial culture.	challenge
updatedbyinstructor	BPPHE:A8E261	2017-04-23 23:26:39 CDT	GO:0008745 N-acetylmuramoyl-L-alanine amidase activity (F)	PMID:21097580	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5: It was previously proven, in the same paper, that orf9 is a N-acetylmuramoyl-L-alanine amidase, but it wasn't known what regions of orf9 was imporant for the lytic activity. To determine the regions of orf9 that are functionally important the researchers constructed eight mutants of the orf9 (of E. faecalis phage φEF24C) by deleting the N-terminal and C-terminal domains (Figure 1A). Only three of the C-terminal deleted mutants were viable (they were soluble), but none of the N-terminal regions were soluble which would indicate that the N-terminal region is required for stability. The C-terminal mutants had their lytic activities were tested by measuring the turbidity, but only the full length orf9, with a Histidine tag, displayed lytic activity. This demonstrated that the full length, N-terminal and C-terminal, orf9 is required for solubilty and lytic activity.	challenge

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Annotations challenged by Canova1

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
updatedbyinstructor	Robins3, Team Green-B	9CAUD:W0TUZ2	GO:0098024 - virus tail, fiber (C)	PMID:27422842	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 3

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