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User:Cait17

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Phage Hunters Spring 2016

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
flagged9CAUD:H9NAL32016-04-04 08:17:10 CDTGO:0004175 endopeptidase activity (F)PMID:22416675ECO:0000314 direct assay evidence used in manual assertion

Figure 4, data not shown.

challenge
requireschanges9CAUD:U5PWR92016-04-06 11:53:54 CDTGO:0004175 endopeptidase activity (F)GO_REF:0000100ECO:0000250 sequence similarity evidence used in manual assertion

The NCBI BlastP shows protein sequence similarity between the protein encoded by the Spock_55 gene in Bacillus phage Spock and the protein encoded by the LysB4 gene (also called BCB4_0006) in B. cereus phage B4.

BlastP values are 100% Query cover, E value = 0.0, and Identity = 99%. It has been demonstrated that the LysB4 protein (produced by the LysB4 gene) exhibits endopeptidase activity in B. cereus phage B4. Therefore it can be inferred that this homologous protein exhibits the same function in Bacillus phage Spock.

challenge
updatedbyinstructor9CAUD:A0A075M0872016-04-06 11:54:38 CDTGO:0004175 endopeptidase activity (F)GO_REF:0000100ECO:0000250 sequence similarity evidence used in manual assertion

The NCBI BlastP shows protein sequence similarity between protein encoded by the LD11_gp054 gene in Bacillus phage Riley and the protein encoded by the LysB4 gene (also called BCB4_0006) in B. cereus phage B4.

BlastP values are 100% Query cover, E value = 8e-179, and Identity = 95%. It has been demonstrated that the LysB4 protein (produced by the LysB4 gene) exhibits endopeptidase activity in B. cereus phage B4. Therefore it can be inferred that this homologous protein exhibits the same function in Bacillus phage Riley.

challenge
unacceptable9CAUD:S5Y0K12016-04-06 11:49:40 CDTGO:0004175 endopeptidase activity (F)other:NCBI BlastPECO:0000250 sequence similarity evidence used in manual assertion

The NCBI BlastP shows protein sequence similarity between the protein encoded by gene 59 in Bacillus phage Troll and the protein encoded by the LysB4 gene (also called BCB4_0006) in B. cereus phage B4.

BlastP values are 100% Query cover, E value = 8e-164, and Identity = 94%. It has been demonstrated that the LysB4 protein (produced by the LysB4 gene) exhibits endopeptidase activity in B. cereus phage B4. Therefore it can be inferred that this homologous protein exhibits the same function in Bacillus phage Troll.

challenge
updatedbyinstructor9CAUD:U5PRL92016-04-06 11:51:37 CDTGO:0004175 endopeptidase activity (F)GO_REF:0000100ECO:0000250 sequence similarity evidence used in manual assertion

The NCBI BlastP shows protein sequence similarity between the protein encoded by the BigBertha_57 gene in Bacillus phage BigBertha and the protein encoded by the LysB4 gene (also called BCB4_0006) in B. cereus phage B4.

BlastP values are 81% Query cover, E value = 4e-101, and Identity = 77%. It has been demonstrated that the LysB4 protein (produced by the LysB4 gene) exhibits endopeptidase activity in B. cereus phage B4. Therefore it can be inferred that this homologous protein exhibits the same function in Bacillus phage BigBertha.

challenge

acceptable:0
unacceptable:1
requires_changes:1
flagged:1

Annotations challenged by Cait17

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
unacceptableCait17,
Team Red B
9CAUD:S5Y0K1GO:0004175 - endopeptidase activity (F)other:NCBI BlastPECO:0000250 sequence similarity evidence used in manual assertionchallengeC: 2
unacceptableMnguy1,
Team Blue B
BACCE:R8CUT3GO:0004604 - phosphoadenylyl-sulfate reductase (thioredoxin) activity (F)PMID:7588765ECO:0000250 sequence similarity evidence used in manual assertionchallengeC: 1
unacceptableCardosa1,
Team Blue B
9CAUD:A0A0K2CZY5GO:0051131 - chaperone-mediated protein complex assembly (P)PMID:15469818ECO:0000250 sequence similarity evidence used in manual assertionchallengeC: 2
unacceptableAmariam1,
Team You can't B. cereus
9CAUD:A0A0K2D047GO:0098025 - virus tail, baseplate (C)other:BlastpECO:0000250 sequence similarity evidence used in manual assertionchallengeC: 2
unacceptableJheins2,
Team Green B
9CAUD:A0A024B3P4GO:0004514 - nicotinate-nucleotide diphosphorylase (carboxylating) activity (F)PMID:8561507ECO:0000250 sequence similarity evidence used in manual assertionchallengeC: 1

0 annotations fixed by Cait17

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