User:Burkewi4

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	PHOLL:Q7N5I7	2014-04-03 14:20:52 CDT	GO:0052043 modification by symbiont of host cellular component (P)	PMID:24188660	ECO:0000314 direct assay evidence used in manual assertion	It was inferred from figure 4 in the article that the amino acids encoded by PLU1961 play a key role in cytotoxicity when combined with the amino acids encoded by PLU1962. When injected individually into cells there was little to no microtuble deploymerization, and this could be seen via confocal microscopy.	challenge
updatedbyinstructor	PHOLL:Q7N5I6	2014-04-03 14:33:16 CDT	GO:0052039 modification by symbiont of host cytoskeleton (P)	PMID:24188660	ECO:0000314 direct assay evidence used in manual assertion	It was inferred from figure 4 in the article that the amino acids encoded by plu1962 play a key role in the effectiveness of the cytotoxicity. When a sample was injected without the plu1962 there was no microtubule depolymerization. When only injected with plu1962 amino acids, there was microtubule depolymerization but not to the same extent as when plu1962 and plu1961 amino acids were injected. All this was observable by confocal microscopy.	challenge
updatedbyinstructor	PHOAA:B6VMP1	2014-04-03 19:17:12 CDT	GO:22615559 positive regulation of protein secretion (P)	PMID:3355079	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4	challenge
updatedbyinstructor	PHOLL:Q7N7Y9	2014-04-03 19:33:29 CDT	GO:0046930 pore complex (C)	PMID:23515159	ECO:0000314 direct assay evidence used in manual assertion	From figure 2 in the paper we can see from various microscopic images of stained samples that the TcdA1 pore formation. In addition through the use of cryoelectron microscopy we are able to see the structure of the TcdA1, which indeed resembles a syringe or a type IV secretion complex.	challenge
unacceptable	PHOTE:T0PH26	2014-04-03 20:44:54 CDT	GO:0000988 protein binding transcription factor activity (F)	PMID:22151606	ECO:0000315 mutant phenotype evidence used in manual assertion	according to figure 2 the knockout mutant showed defective percent of transmission compared to both the wild type and the knockout mutant with reinserted HdfR	challenge

acceptable:0
unacceptable:1
requires_changes:0
flagged:0

Annotations challenged by Burkewi4

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Burkewi4, MichSt14A 28	PHOTE:T0PH26	GO:0000988 - obsolete transcription factor activity, protein binding (F)	PMID:22151606	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2

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