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User:Brittanybloch

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CACAO Spring 2017

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorMYCTU:PKNA2017-04-02 19:51:15 CDTGO:0044117 growth of symbiont in host (P)PMID:25713147ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4B: Graph shows similar cfu counts for the wild type Mycobacterium tuberculosis H37Rv, the complemented (pknA and pknB) strain, and pknB strain at 24 hours. The wild type and complemented strain expressed increased growth at both 4 and 8 weeks while the cfu counts for the pknB strain was below the detection range at both 4 and 8 weeks post infection.

Figure 6C: Shows a growth analysis through absorbance readings over the course of three hours where the pknA strain grew and the pknA mutant showed no growth nor growth recovery. Figure 6D: When plated on ATc (anhydrotetracycline) plates without pristinamycin the complemented strain grew while the pknA mutant and pknB strain did not grow.

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Annotations challenged by Brittanybloch

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorMCGU2014,
Team Lily Whale
YEAST:TRM1GO:0004809 - obsolete tRNA (guanine-N2-)-methyltransferase activity (F)PMID:2426253ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 6

fixed by TheBockster

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