User:Amandajohnson

CACAO Spring 2017

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	STRPY:W0S3I8	2017-03-24 21:43:56 CDT	GO:0045892 negative regulation of transcription, DNA-templated (P)	PMID:27349341	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3B and 3C: Streptococcus pyogenes serotype M1(MGAS5005) with mutation in rocA promoter showed increased transcription of nga and sda1 virulence genes	challenge
updatedbyinstructor	MYCS2:A0QNG2	2017-04-07 17:01:02 CDT	GO:0044117 growth of symbiont in host (P)	PMID:25713147	ECO:0000315 mutant phenotype evidence used in manual assertion	In Mycobacterium smegmatis mc2 155, strain mc(2)ΔpknA strain was transformed with pNit vector, pNit-PknA or pNit-PknAK42M. When the inducer was absent the mc(2)ΔpknA:pNit-PknA grew robustly which indicated wild type PknA was capable of functional complementation. Fig 6C shows the growth of M. smegmatis when transformed and showed that PknA is significant for cell growth. When the PknA kinase activity was absent, seen in Fig 6C with pNit vextor and kinase inactive PknA(K42M), there was no growth recovery. Fig 6E and F demonstrated that PknA is necessary for growth. Depletion of PknA in the mc(2)ΔpknA strain resulted in compromised growth, show in Fig 2B.	challenge

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Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
updatedbyinstructor	Amandajohnson, Team Moldevort	STRPY:W0S3I8	GO:0045892 - negative regulation of DNA-templated transcription (P)	PMID:27349341	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2 fixed by CSears

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