GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
Cacao
| GO:0046158 | ocellus pigment metabolic process | PMID:22234890 | IDA: Inferred from Direct Assay | P | ||||
| This annotation made on page: HUMAN:A8KAQ8 By: Gandii2 (group Team Protein Titans) on 2014-04-06 10:47:06 CDT. | ||||||||
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| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment |
|---|---|---|---|---|
| Challenge | Cermakme, MichSt14A 5 | 2014-04-13 14:15:01 CDT | I would change the notes to explain Figures 4A and 4B as well since these describe how the HERC2 gene and OCA2 expression are related by HLFT binding. Figure 4A: ChIP-qPCR found that HLFT has better binding with the T-allele of HERC2 r12913832 (found in HEMn-DP cells) than the C-allele found in HEMn-LP cells. Figure 4B: Overexpression of HLFT caused increased OCA2 expression only in HEMn-DP cells. The increased OCA2 expression leads to increased pigmentation. | 0 |
| Challenge | Obeykimb, MichSt14A 41 | 2014-04-13 14:12:49 CDT | The notes are not very clear on how exactly this provides proof for this protein. I have made new notes with a different figure. Notes: The HERC2 enhancer is controlled by several transcription factors. Figure 4A-D shows ChIP-qPCR data of the mRNA expression of the different enhancers. Human melanocytes with a SNP rs12913832 of a C to T-allele are more darkly pigmented. Cells with the C-allele and Cells with the T-allele SNP were compared and mRNA expression of the enhancers was measured. The T-allele SNP having cells produced significantly larger amounts of mRNA expression for the enhancers suggesting that this protein plays a role pigmentation. | 0 |
| Public Assessment | JimHu | 2014-05-02 17:58:28 CDT | Note that there are other accepted annotations to this paper. This paper is not about the function of the HERC2 gene. It shows that a SNP in the HERC2 gene creates an enhancer that regulates expression of OCA2. Regulation of transcription from that enhancer is controlled by other proteins. | Unacceptable ✗ Protein ✔ Publication ✔ Qualifier ✗ Go term ✔ Evidence ✔ With/From ✔ Notes ✔ Unique/Original |
| Public Assessment | DanielRenfro | 2014-04-13 14:15:01 CDT | This annotation has been flagged because it has been edited since last assessment ||GO:0046158||ocellus pigment metabolic process||PMID:22234890||IDA: Inferred from Direct Assay||||P||The over-expression of the transcription factor MITF in HEMn-LP cells (cells with the C-allele, which have lighter pigmentation)led to increased OCA2 expression, which darkened the pigmentation of the eye (Figure 4-E).||complete | Flagged |
| Public Assessment | DanielRenfro | 2014-04-13 14:12:49 CDT | This annotation has been flagged because it has been edited since last assessment ||GO:0046158||ocellus pigment metabolic process||PMID:22234890||IDA: Inferred from Direct Assay||||P||The over-expression of the transcription factor MITF in HEMn-LP cells (cells with the C-allele, which have lighter pigmentation)led to increased OCA2 expression, which darkened the pigmentation of the eye (Figure 4-E).||complete | Flagged |
| Private Assessment | Suzialeksander | 2014-04-09 15:46:17 CDT | You need to be an instructor to view these notes. | Requires Changes ✔ Protein ✔ Publication ✔ Qualifier ✔ With/From ✗ Notes |
