GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
Cacao
| GO:0015193 | L-proline transmembrane transporter activity | PMID:22139509 | IDA: Inferred from Direct Assay | F | ||||
| This annotation made on page: BACSU:PUTP By: Adkinstr (group MichSt14A 24) on 2014-04-01 12:37:33 CDT. | ||||||||
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| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment |
|---|---|---|---|---|
| Challenge | Reillyer, MichSt14A 20 | 2014-04-07 12:26:03 CDT | Figure 8A displays the expression of putB-treA reporter gene fusion as a result of PutP or the PutP-related OpuE transporter mutants. Expression of the putB-treA reporter gene fusion remained l-proline inducible in both mutant strains which means that proline-mediated induction of put expression does not depend on PutP- or OpuE-catalyzed proline uptake. This would change the evidence code to IMP. | 0 |
| Public Assessment | Sandyl 27 | 2019-05-02 11:55:19 CDT | Move Annotation 9680 from BACIU:J7JIE8 to BACSU:PUTP | Updated by Instructor |
| Private Assessment | JimHu | 2014-04-21 09:51:14 CDT | You need to be an instructor to view these notes. | Acceptable ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✔ Notes ✔ Unique/Original |
| Public Assessment | DanielRenfro | 2014-04-07 12:26:03 CDT | This annotation has been flagged because it has been edited since last assessment ||GO:0015193||L-proline transmembrane transporter activity||PMID:22139509||IMP: Inferred from Mutant Phenotype||||F||Table 3: The PutP+ strain BLOB9 exhibits high-affinity l-proline transport activity (Km, about 8 μM) with a rather modest capacity (Vmax, about 29 nmol min−1 mg protein−1) in cells that were grown in SMM. However, precultivation of this strain in SMM in the presence of 1 mM l-proline increased l-[14C]proline uptake activity by about 6-fold (Vmax, about 158 nmol min−1 mg protein−1) without influencing the substrate affinity (Km) of the PutP transport system.||complete | Flagged |
| Private Assessment | Ratiujer | 2014-04-03 12:21:31 CDT | You need to be an instructor to view these notes. | Requires Changes ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✗ Notes ✔ Unique/Original |
