GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
Cacao
| GO:0023033 | signaling pathway | 22130678:22130678 | IMP: Inferred from Mutant Phenotype | P | ||||
| This annotation made on page: VIBAL:D0WWP7 By: Hardin57 (group MichSt14A 9) on 2014-03-31 13:40:26 CDT. | ||||||||
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| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment |
|---|---|---|---|---|
| Challenge | Hardin57, MichSt14A 9 | 2014-04-07 18:29:23 CDT | Defending: The evidence code was marked correct in the previous assessment. It was also indicated that the issue was with the notes, which were amended shortly after assessment. The evidence code rules state that IMP trumps IDA. I marked this as IMP because mutants were compared to the wild type in figure 4 to assess LuxT function. | 0 |
| Challenge | Huverdav, MichSt14A 36 | 2014-04-07 17:02:14 CDT | Evidence Code=IDA: Inferred from direct assay | 0 |
| Private Assessment | Suzialeksander | 2014-05-14 12:44:12 CDT | You need to be an instructor to view these notes. | Updated by Instructor |
| Private Assessment | Shabnam | 2014-04-21 15:36:25 CDT | You need to be an instructor to view these notes. | Requires Changes ✔ Protein ✔ Publication ✔ Qualifier ✗ Go term ✔ With/From ✗ Notes ✔ Unique/Original |
| Public Assessment | DanielRenfro | 2014-04-07 18:29:23 CDT | This annotation has been flagged because it has been edited since last assessment ||GO:0023033||signaling pathway||PMID:22130678||IMP: Inferred from Mutant Phenotype||||P|| Figure 1a and 1b used qRT-PCR and westernblot respectively to show that LuxT is most active during the late stage of the growth phase. These results indicate LuxT is density dependent. Figure 2a and 4 both use a LacZ reporter in a beta-galactosidase assay to show that LuxT is involved in a quorum sensing cascade and is a positive regulator of LuxO and a negative, probably post-transcriptional, regulator of LuxO and is regulated itself by LuxU. ||complete | Flagged |
| Public Assessment | DanielRenfro | 2014-04-07 17:02:14 CDT | This annotation has been flagged because it has been edited since last assessment ||GO:0023033||signaling pathway||PMID:22130678||IMP: Inferred from Mutant Phenotype||||P|| Figure 1a and 1b used qRT-PCR and westernblot respectively to show that LuxT is most active during the late stage of the growth phase. These results indicate LuxT is density dependent. Figure 2a and 4 both use a LacZ reporter in a beta-galactosidase assay to show that LuxT is involved in a quorum sensing cascade and is a positive regulator of LuxO and a negative, probably post-transcriptional, regulator of LuxO and is regulated itself by LuxU. ||complete | Flagged |
| Private Assessment | Amcmillan | 2014-04-01 20:21:13 CDT | You need to be an instructor to view these notes. | Requires Changes ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✗ Notes ✔ Unique/Original |
