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GO:0097428protein maturation by iron-sulfur cluster transferPMID:23589868IDA: Inferred from Direct Assay P
This annotation made on page: MASBA:M9PAS4
By: Cvue4 (group Team Ecoli) on 2013-09-15 15:49:03 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeKohli3,
Team WhiteShyPaulCo
2013-09-20 20:41:23 CDT

Two "sister" GO terms are best for this annotation, since the figure indicates that the protein is found in both the mitochondria AND cytosol (MbNifS-C is localized to the cytosol, while MbNif-M was localized to the mitochondria). GO:0005739 (Mitochondrion) and GO:0005829 (Cytosol) should be used. They are both 'cellular component' terms, which are more appropriate than 'process' terms, which involve movement of another protein.

2
ChallengeKohli3,
Team WhiteShyPaulCo
2013-09-17 20:08:06 CDT

GO term should be simply protein localization (GO:0008104) since the figure establishes that if is found in both the mitochondria AND cytosol (MbNifS-C is localized to the mitochondia, while MbNif-M was localized to the mitochondria). A GO term such as 'mitochondrion localization' is too specific, because it overlooks the fact that Nif is also found in the cytosol.

0
ChallengeDjgulizia,
Team I got Krebs
2013-09-17 19:28:23 CDT

GO:0051646 - mitochondrion localization

0
ChallengeWlee83,
Team Hu Ville
2013-09-15 18:42:54 CDT

He/she did not specifically explain the figure 3. It is indeed from figure 2/3. Figure 2 describes N-terminal presequence-dependent targeting of M. balamuthi NifS-M and NifU-M into S. cerevisiae mitochondria in stained bacteria. Also, it describes the localization of the NIF components in M. balamuthi cellular fractions.

0
Private
Assessment
Suzialeksander2013-12-02 14:51:35 CSTYou need to be an instructor to view these notes.Updated by Instructor
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
Bmcintosh2013-09-23 14:44:04 CDT

Kohli3 gets credit for annotation for suggesting the appropriate GO term (mitochondrion).

Corrected Through Challenges
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2013-09-20 20:41:23 CDT

This annotation has been flagged because it has been edited since last assessment

Flagged
Private
Assessment
Suzialeksander2013-09-20 16:40:43 CDTYou need to be an instructor to view these notes.Requires Changes
Public
Assessment
DanielRenfro2013-09-17 20:08:06 CDT

This annotation has been flagged because it has been edited since last assessment

Flagged
Public
Assessment
DanielRenfro2013-09-17 19:28:23 CDT

This annotation has been flagged because it has been edited since last assessment

Flagged
Private
Assessment
Suzialeksander2013-09-16 12:58:11 CDTYou need to be an instructor to view these notes.Requires Changes
Private
Assessment
Suzialeksander2013-09-16 12:52:45 CDTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original