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Category:Team I got Krebs

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorHUMAN:TERF2Roosheel, Team I got Krebs2013-11-09 11:52:09 CSTGO:0032204 regulation of telomere maintenance (P)PMID:22536324ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 1

challenge
unacceptablePSEAE:Y3287Roosheel, Team I got Krebs2013-11-08 19:51:04 CSTGO:0010628 positive regulation of gene expression (P)PMID:10913088ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 8

challenge
unacceptablePSEAE:Y3287Roosheel, Team I got Krebs2013-11-08 19:47:22 CSTGO:0043580 periplasmic space organization (P)PMID:10913088ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 6

challenge
unacceptablePSEAE:CATARoosheel, Team I got Krebs2013-11-08 19:42:01 CSTGO:0043580 periplasmic space organization (P)PMID:10913088ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 6b

challenge
unacceptableENTFO:F2MTL2Roosheel, Team I got Krebs2013-11-08 19:27:18 CSTGO:0004096 catalase activity (Fig. 1)PMID:22590595IMP: Inferred from Mutant Phenotype
challenge
updatedbyinstructorECOLI:CATERoosheel, Team I got Krebs2013-11-08 19:15:50 CSTGO:0004096 catalase activity (F)PMID:17916790ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 3

Deletions show reduced catalase activity

challenge
updatedbyinstructorMOUSE:FYNRoosheel, Team I got Krebs2013-11-08 18:31:04 CSTGO:1900182 positive regulation of protein localization to nucleus (P)PMID:20142099ECO:0000315 mutant phenotype evidence used in manual assertion

Supplementary figure 3. Figure 4.

challenge
unacceptableECOLI:CLPPDjgulizia, Team I got Krebs2013-11-05 21:15:44 CSTGO:0043335 protein unfolding (P)PMID:21529717ECO:0000314 direct assay evidence used in manual assertion

Figure 4 - ClpP complexes with ClpX to unfold GFP during proteolysis.

challenge
updatedbyinstructorECOLI:CLPXDjgulizia, Team I got Krebs2013-11-05 21:09:41 CSTGO:0004176 ATP-dependent peptidase activity (F)PMID:9573050ECO:0000314 direct assay evidence used in manual assertion

Figure 4

challenge
acceptableECOLI:CLPXDjgulizia, Team I got Krebs2013-11-05 21:08:27 CSTGO:0043335 protein unfolding (P)PMID:21529717ECO:0000314 direct assay evidence used in manual assertion

Figure 4 - ClpX unfolds GFP.

challenge
acceptableECOLI:CLPPDjgulizia, Team I got Krebs2013-11-05 21:04:29 CSTGO:0010498 proteasomal protein catabolic process (P)PMID:9573050ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 - Shows ClpP(-) strains degrade the tagged proteins more slowly than wildtype, in vivo.

challenge
updatedbyinstructorECOLI:CLPPDjgulizia, Team I got Krebs2013-11-05 21:04:28 CSTGO:0004176 ATP-dependent peptidase activity (F)PMID:9573050ECO:0000314 direct assay evidence used in manual assertion

Figure 4 - ClpP complexes with both ClpA and ClpX to degrade SsrA-tagged proteins in vitro.

challenge
updatedbyinstructorECOLI:CLPADjgulizia, Team I got Krebs2013-11-05 20:52:40 CSTGO:0004176 ATP-dependent peptidase activity (F)PMID:9573050ECO:0000314 direct assay evidence used in manual assertion

Figure 4 - ClpA complexes with ClpP to degrade SsrA-tagged protein, in vitro.

challenge
unacceptableECO27:CLPXDjgulizia, Team I got Krebs2013-11-05 20:43:41 CSTGO:0043335 protein unfolding (P)PMID:21529717ECO:0000314 direct assay evidence used in manual assertion

Figure 4 - ClpX unfolds GFP.

challenge
unacceptableECO27:CLPXDjgulizia, Team I got Krebs2013-11-05 20:43:41 CSTGO:0010498 proteasomal protein catabolic process (P)PMID:9573050ECO:0000314 direct assay evidence used in manual assertion

Figure 4 - ClpX works with ClpP in vitro to degrade tagged protein.

challenge
unacceptableECO27:B7UJR0Djgulizia, Team I got Krebs2013-11-05 20:40:41 CSTGO:1901800 positive regulation of proteasomal protein catabolic process (P)PMID:21529717ECO:0000314 direct assay evidence used in manual assertion

Figure 6 - With ClpP in complex with ClpX, slippage of the protein occurs much less and thus the ClpP works to positively regulate the catabolic process of GFP.

challenge
unacceptableECO27:B7UJR0Djgulizia, Team I got Krebs2013-11-05 20:40:41 CSTGO:0010498 proteasomal protein catabolic process (P)PMID:9573050ECO:0000314 direct assay evidence used in manual assertion

Figure 4 - ClpP works with ClpA and ClpX in vitro to degrate SsrA-tagged proteins.

challenge
unacceptableECO27:B7UJR0Djgulizia, Team I got Krebs2013-11-05 20:40:41 CSTGO:1901800 positive regulation of proteasomal protein catabolic process (P)PMID:9573050ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows ClpP- strains degrade the SsrA-tagged proteins more slowly than wild type strain, in vivo.

challenge
unacceptableMOUSE:KDM1ARoosheel, Team I got Krebs2013-11-05 19:35:36 CSTGO:0033235 positive regulation of protein sumoylation (P)PMID:17392792ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 5d

challenge
acceptableDROME:PARPRoosheel, Team I got Krebs2013-10-27 17:27:30 CDTGO:1900182 positive regulation of protein localization to nucleus (P)PMID:24055367ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 3C

challenge
unacceptableMOUSE:ZEB1Roosheel, Team I got Krebs2013-10-22 20:55:49 CDTGO:0033235 positive regulation of protein sumoylation (P)PMID:17392792ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 5g

challenge
acceptableMOUSE:PDGFADjgulizia, Team I got Krebs2013-10-22 20:06:03 CDTGO:0048286 lung alveolus development (P)PMID:23938297ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 - Concentration of PDGF-A is critical for development and maintenance of alveolar structure.

challenge
acceptableMOUSE:SWP70Djgulizia, Team I got Krebs2013-10-09 17:59:48 CDTGO:0008064 regulation of actin polymerization or depolymerization (P)PMID:15542837ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2, SWAP-70 KOs exhibit altered regulation of f-actin filaments.

challenge
acceptableMOUSE:SWP70Djgulizia, Team I got Krebs2013-10-09 17:59:48 CDTGO:0032880 regulation of protein localization (P)PMID:15542837ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3, SWAP-70 KOs display decreased localization of Rac1 and Rac2 to the cell periphery.

challenge
acceptableMOUSE:SWP70Djgulizia, Team I got Krebs2013-10-09 17:59:48 CDTGO:0007204 elevation of cytosolic calcium ion concentration (P)PMID:15542837ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4, SWAP-70 KOs don't see the same rapid elevation in Ca2+ ions within the cell that a WT cell does after stimulation.

challenge
acceptableMOUSE:SWP70Djgulizia, Team I got Krebs2013-10-09 17:59:48 CDTGO:0033633 negative regulation of cell-cell adhesion mediated by integrin (P)PMID:15542837ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 7, SWAP-70 KOs adhere much more than the WT cells; this adhesion was found to be dependent on the integrin LFA-1.

challenge
updatedbyinstructorMOUSE:SWP70Djgulizia, Team I got Krebs2013-10-09 17:59:48 CDTGO:0060754 positive regulation of mast cell chemotaxis (P)PMID:15542837ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1, SWAP-70 KOs display reduced migration of bone marrow mast cells.

challenge
acceptableMOUSE:COF1Djgulizia, Team I got Krebs2013-10-09 16:41:11 CDTGO:0030836 positive regulation of actin filament depolymerization (P)PMID:23921380ECO:0000314 direct assay evidence used in manual assertion

Figure 7 B-C show that when exposed to cofilin, the rate of actin filament depolymerization increases.

challenge
acceptableMOUSE:SWP70Djgulizia, Team I got Krebs2013-10-09 16:36:37 CDTGO:0032233 positive regulation of actin filament bundle assembly (P)PMID:23921380ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5, in vivo analysis displays that SWAP-70 -/- have signifiantly fewer actin filament bundles.

challenge
acceptableMOUSE:SWP70Djgulizia, Team I got Krebs2013-10-09 16:36:37 CDTGO:1902309 negative regulation of peptidyl-serine dephosphorylation (P)PMID:23921380ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 8 A-C shows that in SWAP-70 -/- mutants, the protein "cofilin-1" remains phosphorylated for a longer than in the WT.

challenge
acceptableMOUSE:SWP70Djgulizia, Team I got Krebs2013-10-09 16:36:36 CDTGO:0030835 negative regulation of actin filament depolymerization (P)PMID:23921380ECO:0000314 direct assay evidence used in manual assertion

1E shows that increased concentrations of SWAP-70 lead to delays in actin depolymerization

challenge
acceptableMOUSE:SWP70Djgulizia, Team I got Krebs2013-10-09 16:36:36 CDTGO:0051017 actin filament bundle assembly (P)PMID:23921380ECO:0000314 direct assay evidence used in manual assertion

Fig 3 shows that SWAP-70 bundles actin filaments together in both parallel and anti-parallel manners

challenge
unacceptableMOUSE:RETRoosheel, Team I got Krebs2013-09-24 20:55:13 CDTGO:0043066 negative regulation of apoptotic process (P)PMID:23969857ECO:0000314 direct assay evidence used in manual assertion

Fig. 1G

challenge
unacceptableMOUSE:RETRoosheel, Team I got Krebs2013-09-24 19:27:39 CDTGO:0033690 positive regulation of osteoblast proliferation (P)PMID:23969857ECO:0000314 direct assay evidence used in manual assertion

Fig. 1B

challenge
updatedbyinstructorMOUSE:AMPD3Djgulizia, Team I got Krebs2013-09-13 13:59:36 CDTGO:0046031 ADP metabolic process (P)PMID:23078545ECO:0000315 mutant phenotype evidence used in manual assertion

Table 3 shows increase in ADP levels in AMPD3 knockout mice.

challenge
updatedbyinstructorMOUSE:AMPD3Djgulizia, Team I got Krebs2013-09-13 13:59:36 CDTGO:0046039 GTP metabolic process (P)PMID:23078545ECO:0000315 mutant phenotype evidence used in manual assertion

Table 3 shows decrease in GTP levels in AMPD3 knockout mice.

challenge
updatedbyinstructorMOUSE:AMPD3Djgulizia, Team I got Krebs2013-09-13 13:59:36 CDTGO:0046034 ATP metabolic process (P)PMID:23078545ECO:0000315 mutant phenotype evidence used in manual assertion

Table 3 shows increase in ATP levels in AMPD3 knockout mice.

challenge
acceptableMOUSE:AMPD3Djgulizia, Team I got Krebs2013-09-13 13:43:31 CDTGO:0046034 ATP metabolic process (P)PMID:23911318ECO:0000316 genetic interaction evidence used in manual assertion

Figure 5F, in mouse AMPD2/3 double knockouts, there was a 26% increase in ATP levels.

challenge
acceptableMOUSE:AMPD3Djgulizia, Team I got Krebs2013-09-13 13:43:31 CDTGO:0046039 GTP metabolic process (P)PMID:23911318ECO:0000316 genetic interaction evidence used in manual assertion

In Figure 5f, mice AMPD2/3 double knockouts displayed a 33% decrease in GTP levels.

challenge
acceptableMOUSE:AMPD3Djgulizia, Team I got Krebs2013-09-13 13:43:31 CDTGO:0003876 AMP deaminase activity (F)PMID:23911318ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5C shows significantly decreased AMP Deaminase Activity in AMPD3 knockouts.

challenge
acceptableMOUSE:AMPD2Djgulizia, Team I got Krebs2013-09-13 13:36:11 CDTGO:0003876 AMP deaminase activity (F)PMID:23911318ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5C shows significantly decreased AMP Deaminase Activity in AMPD2 knockouts.

challenge
acceptableMOUSE:AMPD2Djgulizia, Team I got Krebs2013-09-13 13:27:25 CDTGO:0046039 GTP metabolic process (P)PMID:22212473ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1, AMPD2 knockout mice exhibited a significant decrease in GTP levels at the age of 12 or 24 weeks.

challenge
acceptableMOUSE:AMPD2Djgulizia, Team I got Krebs2013-09-13 13:27:25 CDTGO:0046034 ATP metabolic process (P)PMID:22212473ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1, AMPD2 knockout mice exhibited a significant decrease in ATP levels at the age of 12 or 24 weeks.

challenge
acceptableMOUSE:AMPD2Djgulizia, Team I got Krebs2013-09-13 13:17:22 CDTGO:0046039 GTP metabolic process (P)PMID:23911318ECO:0000316 genetic interaction evidence used in manual assertion

In Figure 5f, mice AMPD2/3 double knock outs displayed a 33% decrease in GTP levels.

challenge
acceptableMOUSE:AMPD3Djgulizia, Team I got Krebs2013-09-10 19:49:23 CDTGO:0046033 AMP metabolic process (P)PMID:23078545ECO:0000315 mutant phenotype evidence used in manual assertion

Table 3 shows increase in AMP levels in AMPD3 knockout mice.

challenge
acceptableMOUSE:AMPD3Djgulizia, Team I got Krebs2013-09-10 19:49:23 CDTGO:0034101 erythrocyte homeostasis (P)PMID:23078545ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 shows increased levels of hemolysis based on salt concentrations in AMPD3 knockouts compared to wild-type mice when fasting.

challenge
unacceptableMOUSE:AMPD2Djgulizia, Team I got Krebs2013-09-10 19:17:46 CDTGO:0097018 renal albumin absorption (P)PMID:22212473ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2, AMPD2 knockout mice exhibited increased albumin content in the urine.

challenge
acceptableMOUSE:AMPD2Djgulizia, Team I got Krebs2013-09-10 19:09:08 CDTGO:0046034 ATP metabolic process (P)PMID:23911318ECO:0000316 genetic interaction evidence used in manual assertion

Figure 5F, in mouse AMPD2/3 double knockouts, there was a 26% increase in ATP levels

challenge
acceptableMOUSE:AMPD2Djgulizia, Team I got Krebs2013-09-10 19:09:08 CDTGO:0046033 AMP metabolic process (P)PMID:22212473ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1, AMPD2 knockout mice exhibited a significant increase in AMP levels at the age of 12 or 24 weeks.

challenge
unacceptableHUMAN:AMPD2Djgulizia, Team I got Krebs2013-09-10 19:03:03 CDTGO:0003876 AMP deaminase activity (F)PMID:23911318ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2E, patients with lack of AMPD2 showed loss of deaminase activity

challenge

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Pages in category "Team I got Krebs"

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