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GO:0051092positive regulation of NF-kappaB transcription factor activityPMID:10400814IDA: Inferred from Direct Assay P
This annotation made on page: HV1BR:TAT
By: Jbeasley (group Team Everything Burrito) on 2013-02-10 12:40:30 CST.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeJlieblich,
Team Let's Take This Outside
2013-02-17 14:30:19 CST

The evidence code should be IEP because the gel reatrdation analysis is showing levels of NF-κB complexes.

0
Public
Assessment
JimHu2013-02-19 11:54:26 CST

This is slightly confusing, but the annotation is correct. The experiment uses transfection of wt tat and PKR to assay activation of NF-kappaB, using the gel retardation assay to distinguish the activated transcription factor vs the unactivated form that is normally also present. This is an IDA. PKR could be in the with/from, but we don't support that yet for IDA.

Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2013-02-17 14:30:19 CST

This annotation has been flagged because it has been edited since last assessment

Flagged
Private
Assessment
Bmcintosh2013-02-14 14:28:10 CSTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original