GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
Cacao
| GO:0001618 | viral receptor activity | PMID:15496474 | IDA: Inferred from Direct Assay | F | ||||
| This annotation made on page: HUMAN:CLC4M By: Oherrera (group Team That Will Beat You!!!) on 2011-10-18 21:05:26 CDT. | ||||||||
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| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment |
|---|---|---|---|---|
| Challenge | Oherrera, Team That Will Beat You!!! | 2011-10-25 20:08:26 CDT | I understand why you thought this annotation was incorrect, but the cytoplasmic expression is of N protein, not the L-SIGN protein. They used N protein as a label to see the activity of L-SIGN, which is thought to be a viral receptor. CHO cells do not express L-SIGN, which is why there was no immunofluorescence. The immunofluoroscence in the Clone cells means that L-SIGN has viral receptor activity. The following is from the paper: "Fig. 4 shows cytoplasmic expression of viral N protein in Ͻ1% of clone 2.27 cells but not in control CHO cells. These data indicated that some but not all of the CD209L- expressing cells are susceptible to SARS-CoV infection. In cell lines that transiently express ACE2 cDNA, Ͼ50% can be in- fected by SARS-CoV (9, 10). Thus, CD209L is a less efficient receptor for SARS-CoV than ACE2." | 0 |
| Challenge | Jgorso1, Team Step 2 | 2011-10-24 19:24:04 CDT | Wrong go term. Figure 4 only shows cytoplasmic expression of the protein. You should use GO:0051179 (localization) instead. | 0 |
| Challenge | Jgorso1, Team Step 2 | 2011-10-24 19:23:34 CDT | Wrong go term. Figure 4 only shows cytoplasmic expression of the protein. You should use GO:0051179 (localization) instead. | 0 |
| Private Assessment | Sgouni | 2011-11-22 13:18:33 CST | You need to be an instructor to view these notes. | Acceptable |
