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Cacao

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Contributes toGO:0019564aerobic glycerol catabolic processPMID:25220241ECO:0005510 IMP: Inferred from Mutant PhenotypeBiological Process
Experiment on Yersinia pestis, figure 4, that replaced glpD in genome with defective glpD' allele. Bacteria was grown in HIB media to the logarithmic phase in which intracellular concentrations of glycerol-3-phosphate were measured to show significant increase in glycerol-3-phosphate in normal type cells compared to deletion cells.
complete
This annotation made on page: YERPE:Q93NB6
By: GFurletti (group Team Green A 2019) on 2019-03-08 15:43:31 CST.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Private
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JimHu2019-06-06 16:01:57 CDTYou need to be an instructor to view these notes.Updated by Instructor
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JimHu2019-06-06 16:00:06 CDTYou need to be an instructor to view these notes.Requires Changes
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Ivanerill2019-04-08 17:30:39 CDTNo notes given.Acceptable
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DanielRenfro2019-04-03 09:21:10 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
Contributes to GO:0019662 non-glycolytic fermentation PMID:25220241 ECO:0006049 IMP: Inferred from Mutant Phenotype P Table 2: Two different strains of Y. pestis, KIM6+, with a functional glpD gene, and CO92L, with a 93 bp inframe deletion of the glpD allele, were tested for glycerol fermentation under differing conditions. Glycerol fermentation was visualized by indicator plates. Unmodified strains were utilized as controls, in which KIM6+ was positive for glycerol fermentation and CO92L was negative for glycerol fermentation. Allelic exchange was used to exchange the functional glpD in KIM6+ with the nonfunctional glpD of CO92L. The nonfunctional glpD of CO92L was exchanged with the functional glpD of KIM6+. KIM6+ with nonfunctional glpD and CO92L with the functional glpD showed no indicators of glycerol fermentation. Glycerol fermentation was restored in the mutant KIM6+ with nonfunctional glpD when a plasmid vector of functional glpD derived from KIM6+ was added. This is evidence lack of glpD can disrupt glycerol fermentation.

GO Term non-glycolytic fermentation was chosen due to the function of glpD. glpD in this experiment participates in aerobic glycerol fermentation to help form pyruvate from glycerol which does not involve the use of glycolysis as in glycolysis fermentation.||complete
CACAO 13554

on YERPE:Q93NB6
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Ivanerill2019-03-25 12:21:41 CDT

Please rewrite notes; they can certainly be made much more clear. All the "undergoing" makes thing confusing. The evidence is not correct; the authors are not just knocking out a gene, they are doing more than that to prove the point. Please justify that this is "non-glycolytic fermentation"

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DanielRenfro2019-03-24 19:31:26 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
Contributes to GO:0019662 non-glycolytic fermentation PMID:25220241 ECO:0001175 IMP: Inferred from Mutant Phenotype P For Table 2, Y. pestis strains KIM6+, containing functional glpD gene, and CO92L, containing nonfunctional glpD’ gene, underwent allelic exchange for observation of glycerol fermentation along with controls. KIM 6+ cells underwent glycerol fermentation while KIM6+ glpD’ cells did not. When KIM6+ glpD’ was exposed to glpD it underwent glycerol fermentation. CO92L cells did not undergo fermentation, but when exchanged with the functional glpD gene from KIM6+, they still do not undergo glycerol fermentation. This can conclude glpD plays an important role in glycerol fermentation, but other regulatory mechanisms are in place. complete
CACAO 13554
on YERPE:Q93NB6
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Ivanerill2019-03-13 09:13:58 CDT

Evidence could certainly be more specific. I don't see how figure 4 shows that glpD directly participates in glycerol catabolism. It could be regulating it, or regulating something that regulates it, or changing the synthesis rate for all we know. Notes are OK (to the point and well explained), it just looks like the interpretation is wrong. Reassess GO and ECO terms and justify them.

Please use the cross-linked evidence terms from ECO (i.e. those that end in "used in manual assertion" or "used in automatic assertion" Most instances of evidence you will come across will be of type "used in manual assertion". Terms with "used in automatic assertion" imply that the authors did not make a conscious effort to analyze the results of an experiment, letting an algorithm make the call. For instance, if somebody were to use BLAST to determine that a bunch of proteins are homologous (and hence have the same function as the query) and they do not assess the BLAST results in any way (just accept whatever BLAST returns as significant given a preestablished threshold) that could be thought of as an "automatic assertion".


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Private
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Ivanerill2019-03-09 16:23:58 CSTYou need to be an instructor to view these notes.Requires Changes
Protein
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