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GO:0009007site-specific DNA-methyltransferase (adenine-specific) activityPMID:25187538IDA: Inferred from Direct Assay F
This annotation made on page: 9CAUD:A0A076GD02
By: Sbrewer (group BlueSU18) on 2018-07-14 05:28:18 CDT.



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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Public
Assessment
Ivanerill2018-07-24 12:35:38 CDTNo notes given.Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2018-07-18 12:57:48 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity PMID:25187538 IDA: Inferred from Direct Assay F A sequence comparison with two other phages, Rhizobium gallicum (92% identity) and Sinorhizobium medicae (79% identity), showed that ORF27 encodes an N-6 DNA methyltransferase.

To prove the ORF27 gene product is a methyltransferase, researchers used ORF27 to methylate phage lambda DNA and assessed whether it could be digested by restriction enzymes (Figure 4).

A radioactive methylation assay then showed that the DNA methyltransferase did not methylate other substrates. These findings are consistent with results found from other DNA methyltransferase experiments (Table 3).

complete
CACAO 13297
on 9CAUD:A0A076GD02
Flagged
Public
Assessment
Ivanerill2018-07-17 05:06:33 CDT

You can make the notes a bit easier to follow to fellow undergraduates: "Used IPTG-induced comparative digestion assay and an REase treatment for methylation susceptibility", could be reworded as "used ORF27 to methylate phage lambda DNA and assessed whether it could then be digested by REases (or even better, restriction enzymes)".

Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original