GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

9CAUD:A0A076GD02

From GONUTS
Jump to: navigation, search
Species (Taxon ID) Sinorhizobium phage phiLM21. (1524882)
Gene Name(s) No Information Provided.
Protein Name(s) Methyltransferase (ECO:0000313 with EMBL:AII27779.1)
External Links
UniProt A0A076GD02
EMBL KJ743987
RefSeq YP_009221498.1
GeneID 26737522
KEGG vg:26737522
Proteomes UP000028671
GO GO:0008168
InterPro IPR029063
SUPFAM SSF53335

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0009007

site-specific DNA-methyltransferase (adenine-specific) activity

PMID:25187538[1]

ECO:0000314

F

A sequence comparison with two other phages, Rhizobium gallicum (92% identity) and Sinorhizobium medicae (79% identity), showed that ORF27 encodes an N-6 DNA methyltransferase.

To prove the ORF27 gene product is a methyltransferase, researchers used ORF27 to methylate phage lambda DNA and assessed whether it could be digested by restriction enzymes (Figure 4).

A radioactive methylation assay then showed that the DNA methyltransferase did not methylate other substrates. These findings are consistent with results found from other DNA methyltransferase experiments (Table 3).

complete
CACAO 13297

enables

GO:0016740

transferase activity

GO_REF:0000038

ECO:0000323

imported automatically asserted information used in automatic assertion

UniProtKB-KW:KW-0808

F

Seeded From UniProt

complete

enables

GO:0008168

methyltransferase activity

GO_REF:0000038

ECO:0000323

imported automatically asserted information used in automatic assertion

UniProtKB-KW:KW-0489

F

Seeded From UniProt

complete

involved_in

GO:0032259

methylation

GO_REF:0000038

ECO:0000323

imported automatically asserted information used in automatic assertion

UniProtKB-KW:KW-0489

P

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. Dziewit, L et al. (2014) Molecular characterization of a novel temperate sinorhizobium bacteriophage, ФLM21, encoding DNA methyltransferase with CcrM-like specificity. J. Virol. 88 13111-24 PubMed GONUTS page