GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
| Status | Page | User | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|---|---|---|---|---|---|---|---|
| acceptable | 9CAUD:A0A076GD02 | Sbrewer, BlueSU18 | 2018-07-14 05:28:18 CDT | GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity (F) | PMID:25187538 | ECO:0000314 direct assay evidence used in manual assertion | A sequence comparison with two other phages, Rhizobium gallicum (92% identity) and Sinorhizobium medicae (79% identity), showed that ORF27 encodes an N-6 DNA methyltransferase. To prove the ORF27 gene product is a methyltransferase, researchers used ORF27 to methylate phage lambda DNA and assessed whether it could be digested by restriction enzymes (Figure 4). A radioactive methylation assay then showed that the DNA methyltransferase did not methylate other substrates. These findings are consistent with results found from other DNA methyltransferase experiments (Table 3). | challenge |
