Cacao

	GO:0008081	phosphoric diester hydrolase activity	PMID:25307893	IDA: Inferred from Direct Assay	F
This annotation made on page: BPMD2:VG66 By: AHutchison (group Team Purple A 2018) on 2018-04-01 08:56:08 CDT.

Team	Points
Team Purple A 2018	5
Team Purple B 2018	1

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2018-04-03 22:36:34 CDT

Figure 2D should be discussed with more detail because this goes more into and shows more activity in the substrate production.

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2018-04-14 20:38:35 CDT

No notes given.

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✔ Protein
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2018-04-08 21:50:58 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0008081	phosphoric diester hydrolase activity	PMID:25307893	IDA: Inferred from Direct Assay		F	The gene Gp66 in mycobacteriophage D29 acts as a protein phosphatase. In the time span of 1 hour, protein dephosphorylation was observed when D29Gp66 was present (Fig 2B). Phosphoesterase assay using substrates pNPP, bis-pNPP and TmPP show that D29Gp66 can use both phosophomonoester and diester as substrates, with the catalytic activity for bis-NPP (diester) being ten times higher than pNPP (monoester) (Fig 2C). D29Gp66 appears to function mainly as a phosphodiesterase (Fig 2C). Gp66 has very high 2', 3' cyclic phosphodiesterase activity and no 3', 5' activity (Fig 2D).	complete CACAO 13182

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