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BPMD2:VG66
Contents
| Species (Taxon ID) | Mycobacterium phage D29 (Mycobacteriophage D29). (28369) | |
| Gene Name(s) | 66 | |
| Protein Name(s) | Gene 66 protein
Gp66 | |
| External Links | ||
| UniProt | O64258 | |
| EMBL | AF022214 | |
| PIR | A72808 | |
| RefSeq | NP_046883.1 | |
| ProteinModelPortal | O64258 | |
| GeneID | 1261635 | |
| KEGG | vg:1261635 | |
| Proteomes | UP000002131 | |
| GO | GO:0016787 | |
| Gene3D | 3.60.21.10 | |
| InterPro | IPR004843 IPR029052 | |
| Pfam | PF00149 | |
| SUPFAM | SSF56300 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0004112 |
cyclic-nucleotide phosphodiesterase activity |
ECO:0000314 |
F |
Figure 2 shows phosphoesterase activity of the gene product. Figure 3 compares activity to mutated genes and shows that phosphoesterase activity goes down for mutated genes. |
complete | |||||
| GO:0008081 |
phosphoric diester hydrolase activity |
ECO:0000314 |
F |
The gene Gp66 in mycobacteriophage D29 acts as a protein phosphatase. In the time span of 1 hour, protein dephosphorylation was observed when D29Gp66 was present (Fig 2B). Phosphoesterase assay using substrates pNPP, bis-pNPP and TmPP show that D29Gp66 can use both phosophomonoester and diester as substrates, with the catalytic activity for bis-NPP (diester) being ten times higher than pNPP (monoester) (Fig 2C). D29Gp66 appears to function mainly as a phosphodiesterase (Fig 2C). Gp66 has very high 2', 3' cyclic phosphodiesterase activity and no 3', 5' activity (Fig 2D). |
complete | |||||
|
enables |
GO:0016787 |
hydrolase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Dutta, S et al. (2014) Gp66, a calcineurin family phosphatase encoded by mycobacteriophage D29, is a 2', 3' cyclic nucleotide phosphodiesterase that negatively regulates phage growth. FEMS Microbiol. Lett. PubMed GONUTS page
