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Cacao

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GO:0008081phosphoric diester hydrolase activityPMID:25307893IDA: Inferred from Direct Assay F
This annotation made on page: BPMD2:VG66
By: AHutchison (group Team Purple A 2018) on 2018-04-01 08:56:08 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeJRivera,
Team Purple B 2018		2018-04-03 22:36:34 CDT

Figure 2D should be discussed with more detail because this goes more into and shows more activity in the substrate production.

1
Public
Assessment		Ivanerill2018-04-14 20:38:35 CDTNo notes given.Acceptable
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✔ Notes
✔ Unique/Original
Public
Assessment		DanielRenfro2018-04-08 21:50:58 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0008081 phosphoric diester hydrolase activity PMID:25307893 IDA: Inferred from Direct Assay F The gene Gp66 in mycobacteriophage D29 acts as a protein phosphatase. In the time span of 1 hour, protein dephosphorylation was observed when D29Gp66 was present (Fig 2B). Phosphoesterase assay using substrates pNPP, bis-pNPP and TmPP show that D29Gp66 can use both phosophomonoester and diester as substrates, with the catalytic activity for bis-NPP (diester) being ten times higher than pNPP (monoester) (Fig 2C). D29Gp66 appears to function mainly as a phosphodiesterase (Fig 2C). Gp66 has very high 2', 3' cyclic phosphodiesterase activity and no 3', 5' activity (Fig 2D). complete
CACAO 13182
on BPMD2:VG66
Flagged


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