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Cacao

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GO:0044659cytolysis by virus of host cellPMID:29408864IDA: Inferred from Direct Assay P
This annotation made on page:
By: ALe (group Team Red A 2018) on 2018-03-18 10:54:13 CDT.



TeamPoints
Team Red A 20180




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Private
Assessment		Ivanerill2018-04-15 14:17:58 CDTYou need to be an instructor to view these notes.Requires Changes
✗ Protein
Private
Assessment		Ivanerill2018-04-15 14:17:56 CDTYou need to be an instructor to view these notes.Requires Changes
✗ Protein
Public
Assessment		Ivanerill2018-04-15 14:16:45 CDT

The protein is not correct. Figure 2 demonstrates enzymatic activity (it is an in vitro experiment), not "cytolysis by virus of host cell" since no phage is used. Revise and adapt notes accordingly

Requires Changes
✗ Protein
✔ Publication
✔ Qualifier
✗ Go term
✔ Evidence
✔ With/From
✗ Notes
✔ Unique/Original
Public
Assessment		DanielRenfro2018-03-23 17:08:31 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0044659 cytolysis by virus of host cell PMID:29408864 IDA: Inferred from Direct Assay P The antimicrobial activity was confirmed of PlyE146, a novel phage lysin that targets Gram-negative bacteria, and was monitored over time in a time kill experiment where logarithmic growing E. coli K12 cells were exposed to PlyE146 (400 μg/mL). The killing activity started in less than an hour and became bactericidal at 2 h compared to the control as seen in Figure 4a. In addition, the evident disruption of the cell wall after exposure to 400 μg/mL PlyE146 after 1 hour can be visually observed in the TEM images of Figure 6. Compared to the untreated control cells (left panel), the A. baumannii cell (right panel) has many blotches of white spots that indicates loss of cell wall. The untreated cells are fully dark and intact when compared to the PlyE146-treated cells. complete
CACAO 13127
on ECOLI:LYSD
Flagged
Public
Assessment		Scaruso2018-03-23 10:19:50 CDT

You should make a minor change to the notes. The EDTA portion of your comment does not add to your annotation, even though it is relevant to the gene, but the TEM data in Fig. 6 definitely does. Without Fig. 6, you can only say the cells are being killed (GO:0039633 killing by virus of host cell), but combining Fig. 4 with Fig. 6 shows that it is disrupting the cell wall. I would re-write the notes to reflect that and add a bit of detail, and it should be good.

Requires Changes
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✗ Notes
✔ Unique/Original


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