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Cacao

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GO:0016021integral component of membranePMID:9555893IDA: Inferred from Direct Assay C
This annotation made on page: BACSU:XHLB
By: Shahrk (group Team North Phace) on 2017-04-28 14:14:07 CDT.



TeamPoints
Team North Phace0




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Public
Assessment		DanielRenfro2017-05-11 20:13:27 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0019835 cytolysis PMID:9555893 IMP: Inferred from Mutant Phenotype P Figure 6, contains a graph measuring the OD550 (optical density) over time (hours). The lines on the graph showed various lysis genes. These genes had a combination of mutant strains of xhylA (encodes for putative endolysin), xhlB (encodes for putative holin), or xlyA inject inside of them. According to the graph the lines with with solid triangles (POP1234), solid squares (SK109), and a hallow square (SK110) all decreased in turbidity. Since three of these cultures contained contained strains of either xlyA or xhlB; indicating the bacteria in the culture died due to lysis. This proves holin and endolysin play a major role in lysis. complete
CACAO 12900
on BACSU:XHLB
Flagged
Public
Assessment		DanielRenfro2017-05-09 16:12:14 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0019835 cytolysis PMID:9555893 IDA: Inferred from Direct Assay P Figure 6, contains a graph measuring the OD550 (optical density) over time (hours). The lines on the graph showed various lysis genes. These genes had a combination of mutant strains of xhylA (encodes for putative endolysin), xhlB (encodes for putative holin), or xlyA inject inside of them. According to the graph the lines with with solid triangles (POP1234), solid squares (SK109), and a hallow square (SK110) all decreased in turbidity. All three of these cultures contained contained strains of either xlyA or xhlB; indicating the bacteria in the culture died due to lysis. This proves holin and endolysin play a major role in lysis. complete
CACAO 12900
on BACSU:XHLB
Flagged
Public
Assessment		AAJohnson2017-05-06 08:36:46 CDT

That data don't show this protein is a membrane component. Choose a more appropriate GO term based on the data in the figure. Notes aren't clear: "These genes had a combination of mutant strains inject inside of them." What does that mean? For annotating XhlB, which line on the graph supports function of that protein? How do you know? (Refer to Table 1)

Requires Changes
✗ Go term
✗ Notes


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