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GO:0034291canonical holin activityPMID:22389108IDA: Inferred from Direct Assay F
This annotation made on page: BPT4:HOLIN
By: Shahrk (group Team North Phace) on 2017-04-26 07:44:59 CDT.



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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeKvieira,
Team Drexel 17A
2017-05-09 12:13:12 CDT
GO:0044659 is a GO:ID that better refers to the T protein's lytic activity as represented by Figure 2, because this figure is showing results of lysis. not specifically canonical holin activity.
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DanielRenfro2017-05-11 20:05:24 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0019835 cytolysis PMID:22389108 IMP: Inferred from Mutant Phenotype P Figure 2, plots a graph with A550 on the y axis and time of induction (min) on the x axis. The graph illustrated how the alleles tQ87am and tQ209am contain partial Holin activity. When the two alleles are present in the sample; the turbidity decrease indicating the bacteria has died, due to lysis activity. The alleles tQ87am and tQ209am supports gradual lysis after 40 min and 75 min, respectively. complete
CACAO 12886
on BPT4:HOLIN
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DanielRenfro2017-05-09 12:13:12 CDT

This annotation has been flagged because it has been challenged since last assessment

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AAJohnson2017-05-06 08:28:23 CDT

Fig 2, not 4. IMP trumps IDA. Read the definition for your GO term, it's not a good fit. Select a more appropriate GO term that relates to the data in the figure showing host cell lysis.

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