GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

Cacao

Jump to: navigation, search
GO:0030145manganese ion bindingPMID:22949672IMP: Inferred from Mutant Phenotype F
This annotation made on page: PYRHO:RTCB
By: Alqaffasaa (group Team Trypto-pham) on 2017-04-17 10:32:32 CDT.



TeamPoints
Team Trypto-pham0




You must be logged in to challenge this annotation.

Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Public
Assessment
DanielRenfro2017-05-08 15:18:22 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0008664 manganese ion binding PMID:22949672 IMP: Inferred from Mutant Phenotype F in figure 5(A), activity assay shows how the wild type of RtcB ligates a linear RNA to circular RNA. RtcB assayed and found to ligates the 5'-hydroxyl and 2',3'-cyclic phosphate of a 21-nt RNA. the figure also compare the wild type to three other mutant of RtcB. the three mutants did not form a circular RNA, conforming the wild type function. complete
CACAO 12637
on PYRHO:RTCB
Flagged
Public
Assessment
AAJohnson2017-05-08 14:28:48 CDT

GO terms including binding are forbidden in our competition, and isn't really supported by this figure anyway.

The mutated residues are part of the enzyme active site and mutation prevents formation of the circular RNA form. You should be looking for a GO term having something to do with "3′-P RNA ligation mechanism", as your notes are describing.

Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original