Cacao

	GO:0019835	cytolysis	PMID:12657053	IDA: Inferred from Direct Assay	P
This annotation made on page: BPA18:HOLI By: Ramaswamya (group Team North Phace) on 2017-04-12 10:17:18 CDT.

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2017-05-15 11:53:01 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0019835	cytolysis	PMID:12657053	IMP: Inferred from Mutant Phenotype		P	Holin Annotation Notes: Hol 118(83) is a naturally occurring altered portion of the Holin gene “Hol118” which affects cell lysis timings. Hol118(83) had a truncated N-Terminal (Thus a different start codon then usual) and when translated this portion showed slowed cell lysis. The figure shows decrease in optical density for the Hol118(83) mutant phenotypes. The mutant phenotypes were replaced with different start codons which allowed for an increase rate of cell lysis as compared with the wild type Hol118(83). The actual function of Hol118 in the holin polypeptide chain is to increase the rate of cell lysis so by induction the portion of the N-terminal that was missing in the Hol118(83) seemed to counteract its effects and allow for normal functioning .In conclusion Hol118(83) was a strong antiholin that was already present in the holin genome which could be why in UniProt we see both Holin and Antiholin functions.	complete CACAO 12599

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2017-05-06 07:37:10 CDT

This figure involves evaluation of holin mutants, and IMP trumps IDA.

Notes are probably o.k. What is hol118(83)? What is m14? You could improve your notes by making these things very clear.

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