| Challenge | CSears,
Team Girl, you trypsin | 2017-04-16 14:08:08 CDT | - GO:0045892 negative regulation of transcription, DNA-templated
- Figure 2A: Human angiotensen-(1-7) suppressed MMP-8 mRNA expression in human primary aortic smooth muscle cells also treated with human angiotensen II, as compared to cells treated with human angiotensen II only. IDA.
| 0 |
Private
Assessment | Suzialeksander | 2017-05-01 16:13:31 CDT | You need to be an instructor to view these notes. | Acceptable
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✔ Notes
✔ Unique/Original
|
Public
Assessment | DanielRenfro | 2017-04-23 15:57:42 CDT | This annotation has been flagged because it has been edited since last assessment
| Qualifier |
GO ID |
GO term name |
Reference |
Evidence Code |
with/from |
Aspect |
Notes |
Status
|
|
GO:0043407 |
negative regulation of MAP kinase activity |
PMID:28283184 |
IMP: Inferred from Mutant Phenotype |
|
P |
Organism: Homo sapiens.
Protein Name: Angiotensin-(1-7) (Ang-(1-7)).
Notes: Figure 4A is used.
As the paper states, “the activation of p38 MAPK induced by Ang II was counter-regulated by Ang-(1-7). The MAS receptor antagonist A779 blocked the inhibitory effect of Ang-(1-7) on Ang II-induced p38 MAPK activation.” To be clear, Ang-(1-7) needs the MAS receptor to inhibit the effects Ang II, which explains the effect of the A779 receptor antagonist. The chart indeed shows that MAPK levels decrease by a factor of *NEARLY* two when cells are treated with Ang II and Ang-(1-7) as opposed to just Ang II. Thus, the decrease in MMP-8 levels discussed in the paper (when cells are treated with both proteins as opposed to just Ang II) is likely due to a decrease in MAPK activity. Ang-(1-7) shares a UniProt page with Ang II, even though the two are different entities.||complete
CACAO 12356
| on HUMAN:ANGT
| Flagged
|
Public
Assessment | DanielRenfro | 2017-04-23 14:39:19 CDT | This annotation has been flagged because it has been edited since last assessment
| Qualifier |
GO ID |
GO term name |
Reference |
Evidence Code |
with/from |
Aspect |
Notes |
Status
|
|
GO:0043407 |
negative regulation of MAP kinase activity |
PMID:28283184 |
IDA: Inferred from Direct Assay |
|
P |
Organism: Homo sapiens.
Protein Name: Angiotensin-(1-7) (Ang-(1-7)).
Notes: Figure 4A is used.
As the paper states, “the activation of p38 MAPK induced by Ang II was counter-regulated by Ang-(1-7). The MAS receptor antagonist A779 blocked the inhibitory effect of Ang-(1-7) on Ang II-induced p38 MAPK activation.” To be clear, Ang-(1-7) needs the MAS receptor to inhibit the effects Ang II, which explains the effect of the A779 receptor antagonist. The chart indeed shows that MAPK levels decrease by a factor of *NEARLY* two when cells are treated with Ang II and Ang-(1-7) as opposed to just Ang II. Thus, the decrease in MMP-8 levels discussed in the paper (when cells are treated with both proteins as opposed to just Ang II) is likely due to a decrease in MAPK activity. Ang-(1-7) shares a UniProt page with Ang II, even though the two are different entities. As simple treatment of the cells with the protein in question and measurement of MAPK by Western blotting are used for experimental procedures, the evidence code should be IDA.||complete
CACAO 12356
| on HUMAN:ANGT
| Flagged
|
Private
Assessment | Suzialeksander | 2017-04-21 14:01:50 CDT | You need to be an instructor to view these notes. | Requires Changes
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ With/From
✔ Unique/Original
|
Public
Assessment | DanielRenfro | 2017-04-19 10:37:08 CDT | This annotation has been flagged because it has been edited since last assessment
| Qualifier |
GO ID |
GO term name |
Reference |
Evidence Code |
with/from |
Aspect |
Notes |
Status
|
|
GO:0043407 |
negative regulation of MAP kinase activity |
PMID:28283184 |
IDA: Inferred from Direct Assay |
|
P |
Organism: Homo sapiens.
Protein Name: Angiotensin-(1-7) (Ang-(1-7)).
Notes: Figure 4A is used.
As the paper states, “the activation of p38 MAPK induced by Ang II was counter-regulated by Ang-(1-7). The MAS receptor antagonist A779 blocked the inhibitory effect of Ang-(1-7) on Ang II-induced p38 MAPK activation.” To be clear, Ang-(1-7) needs the MAS receptor to inhibit the effects Ang II, which explains the effect of the A779 receptor antagonist. The chart indeed shows that MAPK levels decrease by a factor of more than two when cells are treated with Ang II and Ang-(1-7) as opposed to just Ang II. Thus, the decrease in MMP-8 levels discussed in the paper (when cells are treated with both proteins as opposed to just Ang II) is likely due to a decrease in MAPK activity. Ang-(1-7) shares a UniProt page with Ang II, even though the two are different entities. As simple treatment of the cells with the protein in question and measurement of MAPK by Western blotting are used for experimental procedures, the evidence code should be IDA.||complete
CACAO 12356
| on HUMAN:ANGT
| Flagged
|
Private
Assessment | Suzialeksander | 2017-04-18 15:49:21 CDT | You need to be an instructor to view these notes. | Requires Changes
|
Public
Assessment | DanielRenfro | 2017-04-16 14:08:08 CDT | This annotation has been flagged because it has been challenged since last assessment
| Flagged
|
Private
Assessment | Suzialeksander | 2017-04-11 17:04:40 CDT | You need to be an instructor to view these notes. | Requires Changes
✔ Protein
✔ Publication
✔ Qualifier
✗ Go term
✗ Evidence
✔ With/From
✗ Notes
✔ Unique/Original
|
Private
Assessment | Sandyl 27 | 2017-03-29 12:04:11 CDT | You need to be an instructor to view these notes. | Flagged
|
