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Cacao
GO:0048450 | floral organ structural organization | PMID:25314222 | IMP: Inferred from Mutant Phenotype | P | ||||
This annotation made on page: BRARP:M4D425 By: MCGU2014 (group Team Lily Whale) on 2017-02-05 15:42:20 CST. |
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Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment |
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Challenge | Ziba0916, Team Girl, you trypsin | 2017-04-22 18:02:39 CDT | BraLTP1 fragment in PBI121s was introduced into Agrobacterium tumefaciens GV3101 by electroporation, and positive clones were selected on on LB agar plates at 37°C, supplemented with appropriate concentration of antibiotics (gentamicin 50 mg L−1, rifampicin 50 mg L−1 and kanamycin 50 mg L−1) and PCR verified. The BraLTP1 was genetically transformed, and the overexpression of a genetically transformed protein that alters a geno or phenotype is known as EC:IGI. | 0 |
Challenge | Ziba0916, Team Girl, you trypsin | 2017-04-22 16:27:35 CDT | EC: IMP: overexpression of WT or mutant causing difference in function from normal function is still IMP. | 6 |
Challenge | Ziba0916, Team Girl, you trypsin | 2017-04-11 13:44:32 CDT | Process Figure 9 shows the effects of overexpression of BraLTP1 on the flower organ structure. Notes have incorrect protein and organism. Organism: Brassica rapa subsp. pekinensis (Chinese cabbage) (Brassica pekinensis) Protein: Uncharacterized on Uniprot, known as Bra011229 in the paper. Entry name on Uniprot:M4D425_BRARP Evidence code is IDA, No mutants are used to compare to wild type. Only overexpression of BraLTP1 resulted in early development of longer carpels and outward bending stamen in the flower. | 2 |
Challenge | CSears, Team Girl, you trypsin | 2017-02-21 13:41:05 CST |
| 0 |
Challenge | CSears, Team Girl, you trypsin | 2017-02-07 13:44:55 CST | GO:0048440 carpel development Figure 9C: flowers overexpressing BraLTP1 show distended carpel in budded flowers as compared to wild type flowers. | None, yet. |
Private Assessment | Suzialeksander | 2017-06-13 13:59:41 CDT | You need to be an instructor to view these notes. | Updated by Instructor |
Public Assessment | Suzialeksander | 2017-06-13 13:58:06 CDT | Move Annotation 12249 from BRANA:NLTP1 to BRARP:M4D425 | Updated by Instructor |
Private Assessment | Suzialeksander | 2017-04-24 15:01:29 CDT | You need to be an instructor to view these notes. | Corrected Through Challenges ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✔ Notes ✔ Unique/Original |
Public Assessment | DanielRenfro | 2017-04-22 18:02:39 CDT | This annotation has been flagged because it has been challenged since last assessment | Flagged |
Public Assessment | DanielRenfro | 2017-04-22 16:27:35 CDT | This annotation has been flagged because it has been challenged since last assessment | Flagged |
Private Assessment | JimHu | 2017-04-21 14:55:51 CDT | You need to be an instructor to view these notes. | Requires Changes ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✗ Evidence ✔ With/From ✔ Notes ✔ Unique/Original |
Public Assessment | DanielRenfro | 2017-04-11 13:44:32 CDT | This annotation has been flagged because it has been challenged since last assessment | Flagged |
Private Assessment | Suzialeksander | 2017-02-22 13:55:54 CST | You need to be an instructor to view these notes. | Requires Changes ✗ Protein ✗ Evidence ✗ Notes |
Public Assessment | DanielRenfro | 2017-02-21 13:41:05 CST | This annotation has been flagged because it has been challenged since last assessment | Flagged |
Private Assessment | Suzialeksander | 2017-02-20 14:35:12 CST | You need to be an instructor to view these notes. | Requires Changes ✗ Protein ✔ Publication ✔ Qualifier ✗ Go term ✗ Evidence ✔ With/From ✗ Notes ✔ Unique/Original |