PMID:25314222

Citation	Liu, F, Xiong, X, Wu, L, Fu, D, Hayward, A, Zeng, X, Cao, Y, Wu, Y, Li, Y and Wu, G (2014) BraLTP1, a lipid transfer protein gene involved in epicuticular wax deposition, cell proliferation and flower development in Brassica napus. PLoS ONE 9:e110272
Abstract	Plant non-specific lipid transfer proteins (nsLTPs) constitute large multigene families that possess complex physiological functions, many of which remain unclear. This study isolated and characterized the function of a lipid transfer protein gene, BraLTP1 from Brassica rapa, in the important oilseed crops Brassica napus. BraLTP1 encodes a predicted secretory protein, in the little known VI Class of nsLTP families. Overexpression of BnaLTP1 in B. napus caused abnormal green coloration and reduced wax deposition on leaves and detailed wax analysis revealed 17-80% reduction in various major wax components, which resulted in significant water-loss relative to wild type. BnaLTP1 overexpressing leaves exhibited morphological disfiguration and abaxially curled leaf edges, and leaf cross-sections revealed cell overproliferation that was correlated to increased cytokinin levels (tZ, tZR, iP, and iPR) in leaves and high expression of the cytokinin biosynthsis gene IPT3. BnaLTP1-overexpressing plants also displayed morphological disfiguration of flowers, with early-onset and elongated carpel development and outwardly curled stamen. This was consistent with altered expression of a a number of ABC model genes related to flower development. Together, these results suggest that BraLTP1 is a new nsLTP gene involved in wax production or deposition, with additional direct or indirect effects on cell division and flower development.
Links	PubMed PMC4196963 Online version:10.1371/journal.pone.0110272
Keywords	Amino Acid Sequence; Brassica napus/physiology; Carrier Proteins/chemistry; Carrier Proteins/genetics; Cell Proliferation; Flowers/physiology; Gene Expression; Gene Expression Regulation, Plant; Genes, Plant; Molecular Sequence Data; Phenotype; Plant Leaves; Sequence Alignment; Waxes/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BRANA:NLTP1		GO:0008283: cell proliferation	ECO:0000315:		P		According to Figure 7, the BraLTP 1 overexpression causes cell proliferation which suggests that the standard state also is involved in cell proliferation	complete CACAO 12248
BRARP:M4D425		GO:0048450: floral organ structural organization	ECO:0000315:		P		Figure 9 shows the effects of overexpression of BraLTP1 (Bra011229) on the flower organ structure.	complete CACAO 12249

References

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