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Cacao

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GO:0034290holin activityPMID:22685398IDA: Inferred from Direct Assay F
This annotation made on page: PEPDI:UTXA
By: LMoore (group Team Bulbasaur) on 2016-09-29 22:50:57 CDT.



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Team Bulbasaur5




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Private
Assessment		Suzialeksander2016-10-12 14:25:09 CDTYou need to be an instructor to view these notes.Acceptable
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✔ Notes
✔ Unique/Original
Public
Assessment		DanielRenfro2016-10-11 20:16:54 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0034291 canonical holin activity PMID:22685398 IDA: Inferred from Direct Assay F Figure 8A-B.

All three potential forms of TcdE (tcdEM1) or only M25 and M27 (tcdEM25) or only M27 (tcdEM27) were used. When expressed in E. coli, the TcdEM1 (pCD311) and TcdEM25 (pCD302) forms induced complete bacterial lysis within five minutes after thermo-induction whereas expression of the TcdEM27 form (pCD322) caused complete lysis only at about 40 min. As was the case for λS protein, lysis induced by expression of any of the putative forms of TcdE required expression of the λ endolysin. However, when expressed in E. coli λCmrΔ(SR), carrying a deletion in holin and endolysin genes, the λS, TcdEM1, TcdME25 and TcdEM27 forms did not cause lysis, indicating that the lysis in E. coli could not be due to over-expression of the λS and TcdE forms.||complete
CACAO 12124

on PEPDI:UTXA
Flagged
Private
Assessment		Suzialeksander2016-10-11 18:57:07 CDTYou need to be an instructor to view these notes.Requires Changes
✔ Protein
✔ Publication
✔ Qualifier
✗ Go term
✗ Evidence
✔ With/From
✔ Unique/Original
Private
Assessment		Suzialeksander2016-10-11 18:37:24 CDTYou need to be an instructor to view these notes.Requires Changes
✗ Go term
Private
Assessment		Min.lorna2016-10-11 17:07:30 CDTYou need to be an instructor to view these notes.Flagged


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