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Cacao

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GO:0090284positive regulation of protein glycosylation in GolgiPMID:23447592IMP: Inferred from Mutant Phenotype P
This annotation made on page: MOUSE:UBE3A
By: PGiovanelli (group Team Onix) on 2016-09-27 18:55:19 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeCSears,
Team Pik-Achoo!
2016-10-18 20:35:33 CDT

A fluorometric assay was used to determine Golgi lumen pH in Ube3a-deficient mouse neurons as compared to the wild type. Figure 5B shows the calibration curve used to convert YFP:CFP intensity ratio to pH; Figure 5G shows the fluorescence intensity and figure 5H shows the pH as calculated using the calibration curve.

6
ChallengeCSears,
Team Pik-Achoo!
2016-10-16 18:08:06 CDT

Figure 5D-E - in cells with knockdown of Ube3a, Golgi lumen pH is increased as compared to control cells

0
ChallengeCSears,
Team Pik-Achoo!
2016-10-04 20:29:11 CDT

Protein sialylation is not regulated by the protein, but is due to the increase in Golgi pH GO:0032847 - regulation of cellular pH reduction IMP Figure 5D-H - pH of Golgi lumen is increased in cells with Ube3a knocked down

None, yet.
Private
Assessment
Suzialeksander2016-11-30 15:11:45 CSTYou need to be an instructor to view these notes.Updated by Instructor
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Private
Assessment
JimHu2016-11-08 17:40:23 CSTYou need to be an instructor to view these notes.Corrected Through Challenges
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2016-10-18 20:35:33 CDT

This annotation has been flagged because it has been challenged since last assessment

Flagged
Private
Assessment
Suzialeksander2016-10-17 15:47:22 CDTYou need to be an instructor to view these notes.Requires Changes
Notes
Public
Assessment
DanielRenfro2016-10-16 18:08:06 CDT

This annotation has been flagged because it has been challenged since last assessment

Flagged
Private
Assessment
Suzialeksander2016-10-12 14:24:24 CDTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2016-10-11 20:20:11 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:1905528 positive regulation of Golgi lumen acidification PMID:23447592 IMP: Inferred from Mutant Phenotype P Figure 5 shows that a lack of Ube3a results in a decrease in golgi lumen acidification. complete
CACAO 12116
on MOUSE:UBE3A
Flagged
Private
Assessment
JimHu2016-10-11 19:53:53 CDTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2016-10-04 20:29:11 CDT

This annotation has been flagged because it has been challenged since last assessment

Flagged
Private
Assessment
Suzialeksander2016-10-04 17:44:28 CDTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Unique/Original