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Category:Team Pik-Achoo!

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorMOUSE:D3YTL8CSears, Team Pik-Achoo!2016-11-20 17:55:19 CSTGO:0031307 integral component of mitochondrial outer membrane (C)PMID:26305500ECO:0000314 direct assay evidence used in manual assertion

Figure 2A - Spire1C is localized at the periphery of the mitochondria. Figure 2D - fluorescence assay demonstrated that the N-terminus of Spire1C is exposed to the cytoplasm while the C-terminus is not

challenge
updatedbyinstructorMOUSE:D3YTL8CSears, Team Pik-Achoo!2016-11-20 16:52:37 CSTGO:0090141 positive regulation of mitochondrial fission (P)PMID:26305500ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4

challenge
updatedbyinstructorMOUSE:D3YTL8CSears, Team Pik-Achoo!2016-11-15 20:44:30 CSTGO:0030041 actin filament polymerization (P)PMID:26305500ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 - cells with overexpression of Spire1C show increased actin assembly on mitochondria as compared to control, while cells with overexpression of mutant Spire1C do not show increased actin assembly on mitochondria as compared to control

challenge
updatedbyinstructorMOUSE:D3YTL8CSears, Team Pik-Achoo!2016-11-15 20:00:39 CSTGO:0007015 actin filament organization (P)PMID:26305500ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 - cells with overexpression of Spire1C show increased actin assembly on mitochondria as compared to control, while cells with overexpression of mutant Spire1C do not show increased actin assembly on mitochondria as compared to control

challenge
updatedbyinstructorMOUSE:D3YTL8CSears, Team Pik-Achoo!2016-11-15 19:55:51 CSTGO:0031307 integral component of mitochondrial outer membrane (C)PMID:26305500ECO:0000314 direct assay evidence used in manual assertion

Figure 2A - Spire1C is localized at the periphery of the mitochondria. Figure 2D - fluorescence assay demonstrated that the N-terminus of Spire1C is exposed to the cytoplasm while the C-terminus is not

challenge
unacceptableHUMAN:SPIR1CSears, Team Pik-Achoo!2016-11-08 20:37:00 CSTGO:0051639 actin filament network formation (P)PMID:26305500ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 - cells with overexpression of Spire1C show increased actin assembly on mitochondria as compared to control, while cells with overexpression of mutant Spire1C do not show increased actin assembly on mitochondria as compared to control

challenge
unacceptableHUMAN:SPIR1CSears, Team Pik-Achoo!2016-11-08 20:31:40 CSTGO:0032233 positive regulation of actin filament bundle assembly (P)PMID:26305500ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 - cells with overexpression of Spire1C show increased actin assembly on mitochondria as compared to control, while cells with overexpression of mutant Spire1C do not show increased actin assembly on mitochondria as compared to control

challenge
unacceptableHUMAN:INF2CSears, Team Pik-Achoo!2016-11-08 20:07:53 CSTGO:0090141 positive regulation of mitochondrial fission (P)PMID:26305500ECO:0000316 genetic interaction evidence used in manual assertion

Figure 6C: Cells overexpressing active INF2 and Spire1C show shortened mitochondria as compared to control, while cells overexpressing INF2 and Spire1C that lacks the KIND domain show elongated, tubular mitochondria. Figure 6D: Cells overexpressing Spire1C treated with INF2 siRNA show elongated mitochondria as compared to cells treated with scrambled siRNA.

challenge
unacceptableHUMAN:SPIR1CSears, Team Pik-Achoo!2016-11-08 20:06:10 CSTGO:0090141 positive regulation of mitochondrial fission (P)PMID:26305500ECO:0000316 genetic interaction evidence used in manual assertion

Figure 6C and 6D - cells with overexpression of either Spire1C or INF2 or both display short mitochondria, while cells with knocked down expression of INF2 or mutant Spire1C display tubular mitochondria

challenge
acceptableHUMAN:SPIR1CSears, Team Pik-Achoo!2016-11-08 19:54:14 CSTGO:0090141 positive regulation of mitochondrial fission (P)PMID:26305500ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4

challenge
acceptableHUMAN:SPIR1CSears, Team Pik-Achoo!2016-11-08 19:49:08 CSTGO:0031307 integral component of mitochondrial outer membrane (C)PMID:26305500ECO:0000314 direct assay evidence used in manual assertion

Figure 2A - Spire1C is localized at the periphery of the mitochondria. Figure 2D - fluorescence assay demonstrated that the N-terminus of Spire1C is exposed to the cytoplasm while the C-terminus is not

challenge
acceptableMOUSE:SIR6CSears, Team Pik-Achoo!2016-10-29 00:57:48 CDTGO:0006476 protein deacetylation (P)PMID:27457971ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3B: In islets with Sirt6 knocked down, acetylation of FOXO1 is increased.

challenge
acceptableCHILA:DEFB1CSears, Team Pik-Achoo!2016-10-29 00:36:33 CDTGO:0051873 killing by host of symbiont cells (P)PMID:14996845ECO:0000314 direct assay evidence used in manual assertion

Figure 6 - bacteria and C. albicans incubated with cBD-1 show decreased survival with increased concentrations of cBD-1.

challenge
acceptableSTREE:H2BJK0CSears, Team Pik-Achoo!2016-10-28 23:58:32 CDTGO:0044651 adhesion of symbiont to host epithelial cell (P)PMID:23115034ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4

challenge
acceptableRAT:F1M7B8CSears, Team Pik-Achoo!2016-10-25 19:21:16 CDTGO:1905528 positive regulation of Golgi lumen acidification (P)PMID:23447592ECO:0000315 mutant phenotype evidence used in manual assertion

A fluorometric assay was used to determine Golgi lumen pH in Ube3a-knockdown rat cells as compared to control. Figure 5B shows the calibration curve used to convert YFP:CFP intensity ratio to pH; Figure D shows the fluorescence intensity and figure 5E shows the pH as calculated using the calibration curve.

challenge
unacceptableHUMAN:SIR6CSears, Team Pik-Achoo!2016-10-01 23:23:52 CDTGO:0000209 protein polyubiquitination (P)PMID:27457971ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4F: expression of Sirt6 increases FOXO1 ubiquitination as compared to mutant Sirt6

challenge
updatedbyinstructorHUMAN:SIR6CSears, Team Pik-Achoo!2016-10-01 23:20:22 CDTGO:0006476 protein deacetylation (P)PMID:27457971ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3D

challenge
acceptableHUMAN:SIR6CSears, Team Pik-Achoo!2016-10-01 23:20:21 CDTGO:1901485 positive regulation of transcription factor catabolic process (P)PMID:27457971ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4B-F: FOXO1 is trafficked from nucleus to cytoplasm in cells expressing Sirt6, followed by degradation in the proteasome. When proteasome activity is inhibited, FOXO1 continues to be trafficked from nucleus to cytoplasm. In cells expressing inactive Sirt6, FOXO1 remains in the nucleus. Sirt6 expression increases ubiquitination and subsequent degradation of FOXO1.

challenge
unacceptableHUMAN:TXLNACSears, Team Pik-Achoo!2016-10-01 22:40:39 CDTGO:1905476 negative regulation of protein localization to membrane (P)PMID:27155659ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5c: cells with elevated expression of alpha-taxilin show decreased binding of insulin to receptors on cell membrane, indicating decreased insulin receptors in the membrane.

challenge
acceptableHUMAN:NF2L2CSears, Team Pik-Achoo!2016-10-01 22:28:23 CDTGO:0010628 positive regulation of gene expression (P)PMID:27155659ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3b: knockdown of Nrf2 prevents tBHQ-induced expression of the IR (insulin receptor) gene

challenge
updatedbyinstructorHUMAN:FOXO1CSears, Team Pik-Achoo!2016-09-17 19:37:03 CDTGO:0045944 positive regulation of transcription from RNA polymerase II promoter (P)PMID:27577745ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6C and D show that shRNA-mediated knockdown of FOXO1 decrease expression of MARCH1. Figure 6G shows that, while insulin is absent, FOXO1 binds to the MARCH1 promoter, and while insulin is present, FOXO1 binding is inhibited.

challenge
acceptableHUMAN:MARH1CSears, Team Pik-Achoo!2016-09-17 19:11:48 CDTGO:0000209 protein polyubiquitination (P)PMID:27577745ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 8C shows that wild-type MARCH1 polyubiquitinates INSR beta, while MARCH1 that is E3-ligase activity-defective does not. Figure 8D demonstrates that, in cells, knockdown of MARCH1 results in a decrease in INSR beta ubiquitination.

challenge

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Pages in category "Team Pik-Achoo!"

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