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GO:0004527exonuclease activityPMID:1324872ISA: Inferred from Sequence Alignment PMID:1324872 F
This annotation made on page: BPSP1:DPOL
By: Warrierrk (group Team Snorlax) on 2016-04-21 21:05:49 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeRayesd,
Team Kendrick Jafars
2016-04-27 10:36:57 CDT

Since you're using ISA you need to specify an accession number in the with field for an experimentally determined polymerase protein that has sequence alignment. In your case, that would be DNA polymerase I of Ecoli Po1l. You have a good publication but you do not specify the identity of the phage (Pol1) that has sequence similarity to polymerase in SPO1.

1
Private
Assessment
Suzialeksander2016-06-27 16:50:50 CDTYou need to be an instructor to view these notes.Unacceptable
Public
Assessment
AAJohnson2016-05-05 11:08:21 CDT

This is an old paper publishing the sequence of SPO1 DNA polymerase. See Alignment of SPO1 vs. E coli Kelnow fragment sequences in Figure 3, and their alignment statistics in the text. It is o.k. to submit ISA code using the data in the paper (see instructions here http://gowiki.tamu.edu/wiki/index.php/evidence_codes) but you will have to make sure E coli Klenow has an exisiting annotation based on wet lab data. Your challenger comment is correct that you need the Klenow fragment Uniprot ID as the "With/from".

There is Figure 4 and good text describing conservation of residues important for exonuclease function that you should use as evidence, also, since you chose the Go term for exonuclease activity.

Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original