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Cacao

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NOTGO:0051131chaperone-mediated protein complex assemblyPMID:15469818ISS: Inferred from Sequence Similarity UniProtKB:O64292 UniProtKB:P03734 P
This annotation made on page: 9CAUD:A0A0K2CZY5
By: Cardosa1 (group Team Blue B) on 2016-04-11 02:48:32 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeIac1,
Team Purple B
2016-04-18 14:38:15 CDT

The notes you wrote suggest wet lab experiments were performed on the tail chaperone protein of TsarBomba. Since it has not yet been performed, you would have to find significant correlation by Blasting the gene/protein and then replacing the reference column with GO_REF:0000100. However, after performing ncbi BLASTP with the query as the protein sequence from the uniprot accession number from the Streptococcus phage Sfi19 and the subject as Bacillus phage Tsarbomba, no significant correlation was found, and thus this GO annotations is inaccurate.

2
ChallengeCait17,
Team Red B
2016-04-15 10:50:26 CDT

The evidence code ISS does not match the description in the notes. If you are making the annotation based on the order of genes in the genome, you should use evidence code IGC (Inferred from Genomic Context). The ISS code specifies that this is a transfer annotation, and to do this you would need to include evidence of sequence homology (including results of a Blast search in the notes)

None, yet.
Public
Assessment
Scaruso2016-05-09 15:21:58 CDT

This should be a transfer annotation using the SEA-PHAGES GO Ref (GO_REF 0000100) and ISS or IGC as the evidence code and the protein of interest's Uniprot ID(s) in the with/from field. Then you should have all of the information in this annotation put in the notes, including the PMID and your justification.

Unacceptable
Qualifier
Go term
Evidence
With/From
Notes