GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

Cacao

Jump to: navigation, search
GO:0004146dihydrofolate reductase activityPMID:26147643IMP: Inferred from Mutant Phenotype F
This annotation made on page: ECOLI:DYR
By: E63 (group Team Blue A) on 2016-04-04 09:47:49 CDT.



TeamPoints
Team Blue A5




You must be logged in to challenge this annotation.

Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeAll5,
Team You can't B. cereus
2016-04-04 09:52:15 CDT

Okie doke. Sorry, next time maybe mention that in the notes section to avoid confusion?

None, yet.
ChallengeIyerav,
Team TRUMP2016
2016-04-04 09:30:16 CDT

You should mention how the occluded ground states of the wild-type and mutant proteins are similar, but also different to the closed excited states. You have done well in explaining how the product is released but you should mention the mutant proteins.

None, yet.
ChallengeE63,
Team Blue A
2016-03-30 16:58:12 CDT

I made this a direct annotation since the scientists arrived at their conclusions for the function of dihydrofolate reductase activity experimentally. I then used this direct annotation to make a transfer annotation to TsarBomba through sequence similarity.

None, yet.
ChallengeAll5,
Team You can't B. cereus
2016-03-30 09:50:53 CDT

This needs to be a transfer annotation since your use IMP.

None, yet.
ChallengeAll5,
Team You can't B. cereus
2016-03-30 09:50:41 CDT

This needs to be a transfer annotation since your use IMP.

None, yet.
Public
Assessment
Scaruso2016-05-09 13:59:09 CDTNo notes given.Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2016-04-04 21:47:50 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0004146 dihydrofolate reductase activity PMID:26147643 IMP: Inferred from Mutant Phenotype F Typically, dihydrofolate reductase (DHFR) catalyzes the NADPH-dependent reduction of dihydrofolate (DHF) to tetrahydrofolate (THF). DHFR acts in a catalytic cycle of 5 different intermediates. In the mutant (by changing Leu 28 to Phe), the dissociation rate of the enzyme:substrate complex increases by 10-20 fold. In addition, the rates of the exchange of the excited closed states are very different in the mutants, despite the similarities found in the ground states of the mutant and wild-types. There are 2 pathways, intrinsic and allosteric pathways that this enzyme can take depending on the environmental conditions. complete
CACAO 11420
on ECOLI:DYR
Flagged