Private
Assessment | JimHu | 2015-11-17 17:12:30 CST | You need to be an instructor to view these notes. | Acceptable
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✔ Notes
✔ Unique/Original
|
Public
Assessment | DanielRenfro | 2015-11-17 13:30:58 CST | This annotation has been flagged because it has been edited since last assessment
| Qualifier |
GO ID |
GO term name |
Reference |
Evidence Code |
with/from |
Aspect |
Notes |
Status
|
|
GO:0052208 |
modification of morphology or physiology of other organism via substance secreted by type IV secretion system involved in symbiotic interaction |
PMID:23950720 |
IDA: Inferred from Direct Assay |
|
P |
Figure 1. TEM1 b-lactamase protein translocation reporter assay. Shows translocation of effector protein BspF (BAB1_1948)
Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells. Shows effector protein BspF (BAB1_01948) translocated by T4SS.
Figure 4. A subset of Bsp effectors target the host secretory pathway. Shows that effector protein BspF (BAB1_1948) targets secretory pathway by colocalization with calnexin a marker of ER.
Figure 5. Ectopic expression of BspF (BAB1_1948) in HeLa cells interferes with the host secretory pathway.
Figure 7. Contribution of BspF (BAB1_1948) to inhibition of host cell secretion during infection.||complete
CACAO 11000
| on BRUA2:Q2YLT8
| Flagged
|
Private
Assessment | JimHu | 2015-11-03 20:15:09 CST | You need to be an instructor to view these notes. | Requires Changes
✗ Go term
✗ Notes
|
Public
Assessment | DanielRenfro | 2015-11-03 15:52:07 CST | This annotation has been flagged because it has been edited since last assessment
| Qualifier |
GO ID |
GO term name |
Reference |
Evidence Code |
with/from |
Aspect |
Notes |
Status
|
|
GO:0042000 |
translocation of peptides or proteins into host |
PMID:23950720 |
IDA: Inferred from Direct Assay |
|
P |
Figure 1. TEM1 b-lactamase protein translocation reporter assay.
Panel A and B shows percentage of shift in fluorescence emission that correlates with protein Brucella putative effector BAB1_1948 being translocated into J774A.1 host cell (murine cell line).
Panel C and D shows representative fluorescence micrographs of protein translocation. Also look figure S1 and S2.
Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells.
Panel A. B. abortus strain 2308 (black bars) or virB9 mutant strain (white bars) expressing the CyaA fusions to the indicated Brucella effector proteins were used to infect J774.A1 cells, and the cAMP level of infected cells was determined.||complete
CACAO 11000
| on BRUA2:Q2YLT8
| Flagged
|
Private
Assessment | Suzialeksander | 2015-10-26 15:28:04 CDT | You need to be an instructor to view these notes. | Requires Changes
✗ Go term
✗ Evidence
✗ Notes
|
Public
Assessment | DanielRenfro | 2015-10-18 18:14:02 CDT | This annotation has been flagged because it has been edited since last assessment
| Qualifier |
GO ID |
GO term name |
Reference |
Evidence Code |
with/from |
Aspect |
Notes |
Status
|
|
GO:0044097 |
protein secretion by the type IV secretion system |
PMID:23950720 |
IDA: Inferred from Direct Assay |
|
P |
Figure 1. TEM1 b-lactamase protein translocation reporter assay.
Panel A and B shows percentage of shift in fluorescence emission that correlates with protein Brucella putative effector BAB1_1948 being translocated into J774A.1 host cell (murine cell line).
Panel C and D shows representative fluorescence micrographs of protein translocation. Also look figure S1 and S2.
Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells.
Panel A. B. abortus strain 2308 (black bars) or virB9 mutant strain (white bars) expressing the CyaA fusions to the indicated Brucella effector proteins were used to infect J774.A1 cells, and the cAMP level of infected cells was determined.||complete
CACAO 11000
| on BRUA2:Q2YLT8
| Flagged
|
Private
Assessment | Suzialeksander | 2015-10-13 18:19:46 CDT | You need to be an instructor to view these notes. | Requires Changes
✔ Protein
✔ Publication
✗ Evidence
✔ With/From
✗ Notes
✔ Unique/Original
|
