Private
Assessment | JimHu | 2015-11-03 20:39:43 CST | You need to be an instructor to view these notes. | Unacceptable
✗ Go term
|
Public
Assessment | DanielRenfro | 2015-11-03 15:51:12 CST | This annotation has been flagged because it has been edited since last assessment
| Qualifier |
GO ID |
GO term name |
Reference |
Evidence Code |
with/from |
Aspect |
Notes |
Status
|
|
GO:0042000 |
translocation of peptides or proteins into host |
PMID:23950720 |
IDA: Inferred from Direct Assay |
|
P |
Figure 1. TEM1 b-lactamase protein translocation reporter assay.
Panel A and B shows percentage of shift in fluorescence emission that correlates with protein Brucella putative effector BAB1_1671 being translocated into 774A.1 host cell (murine cell line).
Panel C and D shows representative fluorescence micrographs of protein translocation. Also look Figure S1 and S2.
Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells.
Panel A. B. abortus strain 2308 (black bars) or virB9 mutant strain (white bars) expressing the CyaA fusions to the indicated Brucella effector proteins were used to infect J774.A1 cells, and the cAMP level of infected cells was determined.||complete
CACAO 10999
| on BRUA2:Q2YRH9
| Flagged
|
Private
Assessment | Suzialeksander | 2015-10-26 15:29:50 CDT | You need to be an instructor to view these notes. | Requires Changes
✗ Go term
✗ Evidence
✗ Notes
|
Public
Assessment | DanielRenfro | 2015-10-18 18:13:24 CDT | This annotation has been flagged because it has been edited since last assessment
| Qualifier |
GO ID |
GO term name |
Reference |
Evidence Code |
with/from |
Aspect |
Notes |
Status
|
|
GO:0044097 |
protein secretion by the type IV secretion system |
PMID:23950720 |
IDA: Inferred from Direct Assay |
|
P |
Figure 1. TEM1 b-lactamase protein translocation reporter assay.
Panel A and B shows percentage of shift in fluorescence emission that correlates with protein Brucella putative effector BAB1_1671 being translocated into 774A.1 host cell (murine cell line).
Panel C and D shows representative fluorescence micrographs of protein translocation. Also look Figure S1 and S2.
Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells.
Panel A. B. abortus strain 2308 (black bars) or virB9 mutant strain (white bars) expressing the CyaA fusions to the indicated Brucella effector proteins were used to infect J774.A1 cells, and the cAMP level of infected cells was determined.||complete
CACAO 10999
| on BRUA2:Q2YRH9
| Flagged
|
Private
Assessment | Suzialeksander | 2015-10-13 18:18:59 CDT | You need to be an instructor to view these notes. | Requires Changes
✔ Protein
✔ Publication
✗ Evidence
✔ With/From
✗ Notes
✔ Unique/Original
|
